MGIEasy PCR-Free DNA Library Prep Set (16 RXN)
Library and Circularization
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MGIEasy PCR-Free DNA Library Prep Kit | 1000013456 |
Adapter | MGIEasy PF Adapters-16(tube)Kit | 1000013460 |
Beads | MGIEasy DNA Clean Beads (8 mL) | 940-001596-00 |
MGIEasy PCR-Free DNA Library Prep Set (96 RXN)
Core library |
MGIEasy PCR-Free DNA Library Prep Kit |
1000013457 |
Adapter | MGIEasy PF Adapters-96 (plate) Kit | 1000013461 |
Beads | MGIEasy DNA Clean Beads (50 mL) | 940-001594-00 |
Features
Compatible with Multiple Species Types
Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva, fresh tissue), mice, rice, E.coli and metagenomics.
| Fast, Simple and Automated Workflow Library preparation can be completed in 3.5 hours with size-selected DNA as input; the workflow is simple and suitable for automated library preparation without the risk of amplicon contamination. |
No Amplification Error Accumulation By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity. | Higher Coverage Uniformity
Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region, promoter and repetitive region.
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Excellent Performance of Variant Detection Compared with traditional WGS (PCR amplification), PCR-free WGS shows higher sensitivity and accuracy of variant detection, especially indels. |
Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva, fresh tissue), mice, rice, E.coli and metagenomics.
Library preparation can be completed in 3.5 hours with size-selected DNA as input; the workflow is simple and suitable for automated library preparation without the risk of amplicon contamination.
By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity.
Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region, promoter and repetitive region.
Compared with traditional WGS (PCR amplification), PCR-free WGS shows higher sensitivity and accuracy of variant detection, especially indels.
Product Specification
Product Name |
MGIEasy PCR-Free DNA Library Prep Set |
Application |
Whole genome sequencing |
The version of Set |
V1.1 |
Configuration | 16 RXN(1000013452) and 96 RXN(1000013453) |
Validity period | 9 month |
Assay Time | ~3.5 hrs |
Input Quantity |
80-200 ng fragment DNA |
Sample types | gDNA |
Species Compatibility |
Human, animals,plants, fungi, bacteria, metagenomics, etc. |
Fragmentation Method | Sonication |
Recommended Insert Size | 350-400 bp |
Platform Compatibility | DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7 |
Recommended Read Length |
SE100, PE100, PE150, PE200, SE400 |
Recommended MGI Process
Application
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Library Preparation
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Sequening
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Analysis
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Related Products
Related Documents
- Materials
MGIEasy PCR-Free DNA Library Prep Set brochure
11000013452,1000013453-MGIEasy PCR-Free DNA Library Prep Set User Manual-A1
This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used. - Demo DataRead Length: PE150 Sample: NA12878 Platform: DNBSEQ-G400/MGISEQ-2000
Demo Data 2
Demo Data 3
Demo Data 4
- SDS