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DNBSEQ-T1+: Sequencing in 24 Hours

DNBSEQ-T1+ is one of the fastest T-level benchtop sequencers globally, developed with MGI's core DNBSEQ™ sequencing technology. T1+ supports dual flow cell concurrent operation, generating up to 1.2 Tb of sequencing data within 24 hours (600 Gb per flow cell, totaling 1.2 Tb for two flow cells). Additionally, T1+ offers an optional built-in bioinformatics module, enabling automated advanced analysis immediately after the sequencing run.

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Features

Performance Parameter

Maximum number of Flow Cells	Flow Cell Type****	Lanes/Flow cell	Effective *Reads/Flow Cell**	Read Lengths	Data Output	Q30**	Q40**	Run Time***
2	FCL	4	2000 M	SE50	100 Gb~200 Gb	>93%	>90%	~7 h
				SE100	200 Gb~400 Gb	>93%	>90%	~9.5 h
				PE100	400 Gb~800 Gb	>93%	>90%	≤17 h
				PE150	600 Gb~1.2 Tb	>93%	>90%	~24 h
	FCM	2	1000 M	SE50	50 Gb~100 Gb	>93%	>90%	~6 h
				SE100	100 Gb~200 Gb	>93%	>90%	~7 h
				PE100	200 Gb~400 Gb	>93%	>90%	≤14 h
				PE150	300 Gb~600 Gb	>93%	>90%	~19.5 h
	FCS	2	500 M	SE50	25 Gb~50 Gb	>93%	>90%	~4.5 h
				SE100	50 Gb~100 Gb	>93%	>90%	~6.5 h
				PE100	100 Gb~200 Gb	>93%	>90%	≤13.5 h
				PE150	150 Gb~300 Gb	>93%	>90%	~19 h
				PE300	300 Gb~600 Gb	>85%	>75%	~35 h
* The effective reads are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library preparation method. The percentage of bases above Q30, Q40 and run time is the average of an internal standard library over the entire run. Actual performance is affected by factors such as sample type, library quality, and insert fragment length. * The sequencing time is the statistical duration from ssCirDNA library to FastQ. FCM and FCS are theoretical estimates of duration. **** FCS: Available in H2 2025. FCS PE300 and FCM: Available in H1 2026.

Application Type	Recommended Data Size	Recommended Read Length	Max. Flow Cells/RUN: 2
			*1FCS**	*1FCM**	*1FCL**
			500 M reads	1000 M reads	2000 M reads
NIPT/PGS	10 M reads/sample	SE50/SE100	40	80	160
Small RNA	25 M reads/sample	SE50	16	32	64
RNA-Seq	25 M reads/sample	SE50	16	32	64
Metagenomics for pathogen detection	20 M reads/sample	SE50/SE100	20	40	80
Single cell RNA-Seq	10K cells, 50K reads/cell, 500 M/sample	PE150	1	2	4
Oncology panel	10 Gb/sample (5000X，1Mb panel)	PE150	12	24	48
Companion Diagnostic Onco panel	1 Gb/sample		120	240	480
Microbial WGS	1 Gb/sample		120	240	480
ATOPlex Panel	Respiratory tract panel/ COVID-19 panel：5M reads/sample		80	160	320
Transcriptomics	6 Gb/sample	PE150	20	40	80
WES	100X average sequencing depth 15 Gb/ sample		8	16	32
WGS	30X average sequencing depth 100 Gb/ sample		1	2	4
WGBS	30X average sequencing depth 120 Gb/sample		1	2	4
Oncology targeted methylation panel	5 Gb/sample (2000X, 0.5Mb panel)		24	48	96
16S	0.1M reads/sample	PE300	1152 (576/lane)	/	/
Recommended data output and sample numbers are only for reference, actual application will require optimisation adjustments.

Simple: DNB Make-Sequencing-Analysis, All-in-One

DNBSEQ-T1+ introduces the new DNB M&L (DNB Make & Load) functional module, integrating DNB preparation and loading directly into the sequencer. It enables high-quality, one-click DNB preparation with results ready for sequencing. And offers three flow cell specifications ("Large-Medium-Small"): FCL, FCM and FCS. Each flow cell can independently run different sequencing read lengths or application types. DNBSEQ-T1+ is equipped with an independent and specific lane loading system and also supports various library types (dsDNA, ssCirDNA and DNB). We offers two configurations for different user groups (DNBSEQ-T1+ & DNBSEQ-T1+A). DNBSEQ-T1+A has a built-in bioinformatics module that can automatically initiate advanced analysis for specific applications.

System Parameter

Power & Dimensions & Net Weight
Power Type: 200 V~240 V，~16A

Rated Power: 2000 W

Dimensions (L×W×H): 1150 mm×750 mm×810 mm

Net Weight: ＜300 kg
Control Computer Configurations
Main Screen: LCD, 21.5 inches, 1920×1080 pixels

Secondary Screen: LCD, 19 inches, 1920×360 pixels
Operating Environment Requirements
Temperature: 15 ℃~30 ℃

Relative humidity: 20% RH~80% RH, non-condensing

Atmospheric pressure: 70 kPa~106 kPa
Storage/Transportation Environment Requirements
Temperature: -20 ℃~50 ℃

Relative humidity: 15% RH~85% RH, non-condensing

Atmospheric pressure: 70k Pa~106 kPa

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DNBSEQ-T1+: Sequencing in 24 Hours

Features

Performance Parameter

System Parameter

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