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DNBSEQ-E25RS High-throughput Sequencing Set

DNBSEQ-E25RS High-throughput Sequencing Set is specifically designed for DNA or RNA sequencing on DNBSEQ-E25RS/ARS. This sequencing set is intended to be used for small genome sequencing, metagenomic sequencing, targeted sequencing and other research applications, but cannot be used for clinical diagnoses.

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DNBSEQ-E25RS High-throughput Sequencing Set (FCL SE100)

Item No.：940-000573-00

940-000570-00	DNBSEQ-E25RS High-throughput Sequencing Kit (FCL SE100)
930-000045-00	DNBSEQ-E25RS Sequencing Flow Cell

DNBSEQ-E25RS High-throughput Sequencing Set (FCL PE150)

Item No.：940-000567-00

940-000571-00	DNBSEQ-E25RS High-throughput Sequencing Kit (FCL PE150)
930-000045-00	DNBSEQ-E25RS Sequencing Flow Cell

DNBSEQ-E25RS High-throughput Sequencing Set (App-C FCL SE100)

Item No.：940-000569-00

940-000572-00

DNBSEQ-E25RS High-throughput Sequencing Kit (App-C FCL SE100)

930-000045-00

DNBSEQ-E25RS Sequencing Flow Cell

DNBSEQ-E25RS High-throughput Sequencing Set (App-C FCL PE150)

Item No.：940-000574-00

940-000568-00

DNBSEQ-E25RS High-throughput Sequencing Kit (App-C FCL PE150)

930-000045-00

DNBSEQ-E25RS Sequencing Flow Cell

Features

Easy to operate DNB loading and sequencing can be done with only one sample injection.	Easy to maintain Reagents enter flow cell directly from reagent cartridge, no instrument wash required between runs.
Simplified sequencing process Sequencing set has one-step DNB making reagent, which can direct access to DNB samples.	Seamless conversion Reagents used for library conversion, one-step DNB making and sequencing cartridge with pre-filled primers are all included, enabling sequencing of third-party libraries without purchasing additional reagents.

Easy to operate

DNB loading and sequencing can be done with only one sample injection.

Easy to maintain

Reagents enter flow cell directly from reagent cartridge, no instrument wash required between runs.

Simplified sequencing process

Sequencing set has one-step DNB making reagent, which can direct access to DNB samples.

Seamless conversion

Reagents used for library conversion, one-step DNB making and sequencing cartridge with pre-filled primers are all included, enabling sequencing of third-party libraries without purchasing additional reagents.

Product Specification

Read length	SE100	PE150	APP-C SE100***	App-C PE150***
Maximum flow cell number	1	1	1	1
Effective reads/flow cell*	25 M	25 M	20 M	20 M
Data output	2.5 Gb	7.5 Gb	2.0 Gb	6.0 Gb
Q30**	>90%	>80%	>90%	>80%
Run time (DNB-FQ)	~5 h	~20 h	~5 h	~20 h
* The effective reads value is obtained according to the operation of a specific standard library, and the actual application library will fluctuate according to the sample type and library construction method. The percentage of bases above Q30 and the running time are averaged over the entire run for a particular standard library. The practical application performance is affected by sample type, library quality, insert length and other factors. * The App-C Sequencing Set includes the invertase module, eliminating the need to purchase additional conversion reagents. **** The existing kit supports SE50 and PE100 reads, and the instrument does not have SE50 and PE100 sequencing modes.