CycloneSEQ-WT02: Nano Gates to the Macro Cosmos

Sequencing Principle

During the CycloneSEQ-WT02 sequencing process, the DNA library molecules linked to the motor protein are drawn to the vicinity of the nanopore protein embedded in the biomimetic membrane under the influence of electric field forces, where they are captured by the nanopore protein. Meanwhile, motor proteins situated near the nanopore proteins entrance, steadily and rapidly unwinding the DNA. This allows the DNA libraries to pass through the nanopore as a single strand. Different DNA bases and their arrangement impede the current to varying degrees, triggering the current fluctuations. The channel sensor captures these current fluctuation data and transmits them to the computer system, where basecalling algorithms parse the information to achieve real-time and accurate gene sequencing.