Microbiological Detection Total Solution
MGI has developed a high-throughput sequencing platform integrated with a pathogen detection system. This innovative technology can perform fast, accu...
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Comprehensive and flexible genome sequencer
DNBSEQ-G400 is built with a new flow cell system that can flexibly support a variety of different sequencing modes. It adopts optimized optical and biochemical systems, which can complete the sequencing process rapidly, giving users a more streamlined sequencing experience.
Features
55GB~1440GB per run
|
PE150(FCS) sequencing at full capacity in only 37 hours |
Supports a range of read lengths, including SE50, SE100, SE400, PE100, PE150, PE200 |
Supports sequencing and data analysis in a wide range of areas, including basic research, clinical research, forensics, and agriculture. |
Related Products
Library preparation
- DNA Sequencing Library Preparation System MGISP-100RS
- High-throughput Automated Sample Preparation System MGISP-960RS
- MGIEasy Cell-free DNA Library Prep Set
- MGIEasy Single Cell Whole Genome Amplification Kit
- MGICare Detection Kit for Chromosome Copy Number Variation Test
- MGIEasy Rapid DNA Library Prep Kit customized for CDC
Sequencing
- DNBSEQ-G400RS High-throughput Sequencing Set (SE50)
- DNBSEQ-G400RS High-throughput Sequencing Set (SE100)
- DNBSEQ-G400RS High-throughput Sequencing Set (SE400)
- DNBSEQ-G400RS High-throughput Sequencing Set (PE100)
- DNBSEQ-G400RS High-throughput Sequencing Set (PE150)
- DNBSEQ-G400RS High-throughput Sequencing Set (PE200)
- DNBSEQ-G400RS High-throughput Rapid Sequencing Set (SE100)
- DNBSEQ-G400RS High-throughput Rapid Sequencing Set (PE100)
- DNBSEQ-G400RS High-throughput Rapid Sequencing Set (PE150)
Performance Parameter
Q30≥85%（The percentage of base above Q30 is the average of an internal standard library over the entire run. ）
| DNBSEQ-G400 Specifications | ||
|---|---|---|
| Platform | DNBSEQ-G400 | |
| Flow Cell No. | 2 | |
| Flow Cell Type | FCS | FCL |
| Lane No./Flow Cell | 2 | 4 |
| Effective Reads/Flow Cell* | 550M | 1500-1800M |
|
Read Lengths |
SE100 PE100 PE150 |
SE50 SE100 SE400 PE100 PE150 PE200 |
| * The maximum number of effective reads are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library preparation method. | ||
| DNBSEQ-G400RS Performance | |||
|---|---|---|---|
| Read Lengths | Data Output/Flow Cell | Data QualityQ30* | Run Time** |
| SE50-FCL | 75-90G | >85% | ~14h |
| SE100-FCL | 150-180G | >85% | ~25h |
| SE400-FCL | 600-720G | >70% | ~109h |
| PE100-FCL | 300-360G | >85% | ~48h |
| PE150-FCL | 450-540G | >75% | ~66h |
| PE200-FCL | 600-720G | >75% | ~107h |
| SE100-FCS | 55G | >85% | ~13h |
| PE100-FCS | 110G | >85% | ~26h |
| PE150-FCS | 165G | >75% | ~37h |
| * The percentage of base above Q30 is the average of an internal standard library over the entire run. The actual performance is affected by factors such as sample type, library quality, and insert fragment length. | |||
| ** Run time was calculated based on dual-slides mode, and includes sample loading, sequencing, base calling and data processing. | |||
System Parameter
- Operating Environment Requirements
Temperature：19-25℃
Relative Humidity：20% RH-80% RH，non-condensing
Atmospheric Pressure：70-106 kPa
Waterproof Rating：IPX0
- Control Computer Configurations
CPU：Intel Xeon E5 10Core x 2 2.2GHz
Internal Storage：256GB RAM
HDD：16TB
SSD：480G
Operating System：Windows 10 Enterprise
- Power&Dimensions&Net Weight
Dimensions：Length：1086mm；Width：756mm；Height：710mm
Net Weight：200kg
Power Type：100~240V，50/60Hz
Rated Power：1200VA
- Product Model*
DNBSEQ-G400
DNBSEQ-G400RS (for research only)
DNBSEQ-G400CX (clinical purpose, overseas IVD)
*Remark: DNBSEQ-G400 remain their original product names as MGISEQ-2000 respectively in China and some other overseas countries.
Related Documents
- Materials
DNBSEQ-G400RS High-throughput (Rapid) Sequencing Set User Manua-A2
DNBSEQ-G400RS CoolMPS High-throughput Sequencing Set User Manual-A0
DNBSEQ-G400 High-throughput Sequencing Set User Manual-A1
DNBSEQ-G400RS High-throughput Sequencing Set (stLFR) User Manual-A3
- Webinar
MGI DNBSEQ™ Sequencing Technology
Design of experiments and WGS library preparation workflow
Quality Control for DNBSEQ Platforms and Best Practices for DNBSEQ-G400RS
- Videos
1-Best Practice of DNBSEQ-G400_DNB Generation
2-Best Practice of DNBSEQ-G400_DNB Loading
