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MGIEasy FS PCR-Free DNA Library Prep Set

MGIEasy FS PCR-Free DNA Library Prep Set is specifically designed for constructing WGS libraries without PCR amplification for the MGI high-throughput sequencing platform. For the high-quality and low-bias fragmentation enzyme, the set could fragment 50-1000 ng gDNA. The distribution of fragmented DNA is similar to that fragmented by mechanical techniques. The set combined with the automatic sample preparation system MGISP, could provide an automatic library preparation workflow. FS PCR-free combined with MGI DNBSEQTM sequencer, is truly PCR-free sequencing with no amplification errors accumulation.


MGIEasy FS PCR-Free DNA Library Prep Set (96 RXN)

Item No.:1000013455
Library and Circularization MGIEasy FS PCR-Free DNA Library Prep Kit
1000013459
Adapter MGIEasy PF Adapters-96(plate)Kit 1000013461
Beads MGIEasy DNA Clean Beads

1000005279

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MGIEasy FS PCR-Free DNA Library Prep Set (16 RXN)

Item No.:1000013454
Library and Circularization MGIEasy FS PCR-Free DNA Library Prep Kit 1000013458
Adapter

MGIEasy PF Adapters-16(tube)Kit

1000013460
Beads MGIEasy DNA Clean Beads 1000005278
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Features

Good compatibility for different species fragmentation
Good compatibility for different species fragmentation
Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the consistent and concentrated fragment range.
Stable library yield
Stable library yield
The modified reaction system increases the library quality and results in a stable library yield.
Simple and automated workflow
Simple and automated workflow
Library construction can be completed within 3.9 hours. Compatible with automatic sample preparation systems to provide an automated solution.
No amplification error accumulation
No amplification error accumulation
WGS PCR-free library preparation in combination with sequencing by the MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity.
Good coverage uniformity
Good coverage uniformity
The genome coverage of FS PCR-free achieved comparable performance to Covaris PCR-free. The FS PCR-free technique covers the GC-rich, promoter and repetitive regions. Significantly better than traditional WGS methods.
Excellent variant performance
Excellent variant performance
The variant detection performance of FS PCR-free is similar to Covaris PCR-free, and better than traditional PCR, especially InDels.
Good compatibility for different species fragmentation
Good compatibility for different species fragmentation
Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the consistent and concentrated fragment range.
Stable library yield
Stable library yield
The modified reaction system increases the library quality and results in a stable library yield.
Simple and automated workflow
Simple and automated workflow
Library construction can be completed within 3.9 hours. Compatible with automatic sample preparation systems to provide an automated solution.
No amplification error accumulation
No amplification error accumulation
WGS PCR-free library preparation in combination with sequencing by the MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity.
Good coverage uniformity
Good coverage uniformity
The genome coverage of FS PCR-free achieved comparable performance to Covaris PCR-free. The FS PCR-free technique covers the GC-rich, promoter and repetitive regions. Significantly better than traditional WGS methods.
Excellent variant performance
Excellent variant performance
The variant detection performance of FS PCR-free is similar to Covaris PCR-free, and better than traditional PCR, especially InDels.

Product Specification

Product Name MGIEasy FS PCR-Free DNA Library Prep Set
Application Whole genome sequencing
The version of Set

V1.1

Configuration (Cat.) 16 RXN(1000013454), 96 RXN(1000013455)
Validity period 9 months
Assay Time ~3.9 hours
Input Quantity 50-1000 ng gDNA
Sample types gDNA,WGA DNA
Species Compatibility Human, animals, plants, fungi, bacteria, metagenomics, etc.
Fragmentation Method

Enzymatic

Recommended Insert Size 350-660 bp
Platform Compatibility

DNBSEQ-G400, DNBSEQ-G50,DNBSEQ-T7, MGISEQ-2000, MGISEQ-200, BGISEQ-500

Recommended Read Length SE100, PE100, PE150

Recommended MGI Process

Application
Library Preparation
Sequening
Analysis
  • Human WGS sequencing (High depth)

  • DNBSEQ-G400

  • DNBSEQ-G50

  • DNBSEQ-T7

  • MGISEQ-2000

  • MGISEQ-200

  • BGISEQ-500



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