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MGIEasy PCR-Free DNA Library Prep Set

MGIEasy PCR-Free Library Prep Set is specifically designed for making WGS libraries for MGI High-throughput Sequencing Platforms. This library prep set is optimized to convert 80-200 ng size selected fragmented DNA into a customized library. MGI WGS PCR-free library preparation, combined with MGI DNBSEQTM sequencer, is the true PCR-free NGS workflow without PCR amplification, resulting in no error accumulation and higher data accuracy.


MGIEasy PCR-Free DNA Library Prep Set

MGIEasy PCR-Free DNA Library Prep Set (16 RXN)

Item No.:1000013452


Library and Circularization


MGIEasy PCR-Free DNA Library Prep Kit 1000013456
Adapter MGIEasy PF Adapters-16(tube)Kit 1000013460
Beads MGIEasy DNA Clean Beads 1000005278
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MGIEasy PCR-Free DNA Library Prep Set (96 RXN)

Item No.:1000013453

Core library

MGIEasy PCR-Free DNA Library Prep Kit

1000013457
Adapter MGIEasy PF Adapters-96(plate)Kit 1000013461
Beads MGIEasy DNA Clean Beads 1000005279
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Features

Compatible with Multiple Species Types
Compatible with Multiple Species Types


Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva, fresh tissue), mice, rice, E.coli and metagenomics.


Fast, Simple and Automated Workflow
Fast, Simple and Automated Workflow

Library preparation can be completed in 3.5 hours with size-selected DNA as input; the workflow is simple and suitable for automated library preparation without the risk of amplicon contamination.

No Amplification Error Accumulation
No Amplification Error Accumulation

By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity.

Higher Coverage Uniformity
Higher Coverage Uniformity


Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region, promoter and repetitive region.


Excellent Performance of Variant Detection
Excellent Performance of Variant Detection

Compared with traditional WGS (PCR amplification), PCR-free WGS shows higher sensitivity and accuracy of variant detection, especially indels.

Compatible with Multiple Species Types
Compatible with Multiple Species Types


Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva, fresh tissue), mice, rice, E.coli and metagenomics.


Fast, Simple and Automated Workflow
Fast, Simple and Automated Workflow

Library preparation can be completed in 3.5 hours with size-selected DNA as input; the workflow is simple and suitable for automated library preparation without the risk of amplicon contamination.

No Amplification Error Accumulation
No Amplification Error Accumulation

By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity.

Higher Coverage Uniformity
Higher Coverage Uniformity


Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region, promoter and repetitive region.


Excellent Performance of Variant Detection
Excellent Performance of Variant Detection

Compared with traditional WGS (PCR amplification), PCR-free WGS shows higher sensitivity and accuracy of variant detection, especially indels.

Product Specification

Product Name

 MGIEasy PCR-Free DNA Library Prep Set

Application

Whole genome sequencing

The version of Set

V1.1
Configuration 16 RXN(1000013452) and 96 RXN(1000013453)
Validity period 9 month
Assay Time ~3.5 hours
Input Quantity

80-200 ng fragment DNA

Sample types gDNA
Species Compatibility

Human, animals,plants, fungi, bacteria, metagenomics, etc.

Fragmentation Method Sonication
Recommended Insert Size 350-400 bp
Platform Compatibility DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7, BGISEQ-500, MGISEQ-2000, MGISEQ-200
Recommended Read Length SE100, PE100, PE150, PE200,SE400

Recommended MGI Process

Application
Library Preparation
Sequening
Analysis
  • Human WGS sequencing (High depth)

  • DNBSEQ-G400

  • DNBSEQ-G50

  • DNBSEQ-T7

  • BGISEQ-500

  • MGISEQ-2000

  • MGISEQ-200

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