MGIEasy FS PCR-Free DNA Library Prep Set (96 RXN)
Library and Circularization |
MGIEasy FS PCR-Free DNA Library Prep Kit |
1000013459 |
Adapter | MGIEasy PF Adapters-96 (plate) Kit |
1000013461 |
Beads |
MGIEasy DNA Clean Beads (50 mL) |
940-001594-00 |
MGIEasy FS PCR-Free DNA Library Prep Set (16 RXN)
Library and Circularization | MGIEasy FS PCR-Free DNA Library Prep Kit | 1000013458 |
Adapter |
MGIEasy PF Adapters-16 (tube) Kit |
1000013460 |
Beads | MGIEasy DNA Clean Beads (8 mL) | 940-001596-00 |
Features
Good compatibility for different species fragmentation Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the consistent and concentrated fragment range. | Stable library yield The modified reaction system increases the library quality and results in a stable library yield. |
Simple and automated workflow Library construction can be completed within 3.9 hours. Compatible with automatic sample preparation systems to provide an automated solution. | No amplification error accumulation WGS PCR-free library preparation in combination with sequencing by the MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity. |
Good coverage uniformity The genome coverage of FS PCR-free achieved comparable performance to Covaris PCR-free. The FS PCR-free technique covers the GC-rich, promoter and repetitive regions. Significantly better than traditional WGS methods. | Excellent variant performance The variant detection performance of FS PCR-free is similar to Covaris PCR-free, and better than traditional PCR, especially InDels. |
Product Specification
Product Name | MGIEasy FS PCR-Free DNA Library Prep Set |
Application | Whole genome sequencing |
The version of Set |
V1.2 (16RXN), V1.3 (96RXN) |
Configuration (Cat.) | 16 RXN(1000013454), 96 RXN(1000013455) |
Validity period | 9 months |
Assay Time | ~3.9 hrs |
Input Quantity |
50-1000 ng gDNA |
Sample types |
gDNA, WGA DNA |
Species Compatibility | Human, animals, plants, fungi, bacteria, metagenomics, etc. |
Fragmentation Method |
Enzymatic |
Recommended Insert Size |
350-660 bp |
Platform Compatibility |
DNBSEQ-G400, DNBSEQ-G50,DNBSEQ-T7 |
Recommended Read Length | SE100, PE100, PE150 |
Recommended MGI Process
Application
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Library Preparation
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Sequening
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Analysis
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Related Products
Related Documents
- Mateirals
1000013454, 1000013455- MGIEasy FS PCR-Free DNA Library Prep Set FAQ-- A1
1000013454, 1000013455- MGIEasy FS PCR-Free DNA Library Prep Set User Manual
This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used.
- Demo DataRead Length:PE150 Sample:NA12878 Platform:DNBSEQ-G400/MGISEQ-2000
Demo Data 1 Demo Data 2 Demo Data 3 Demo Data 4 Read Length:PE100 Sample:NA12878 Platform:DNBSEQ-T7
Demo Data 1 - SDS