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MGIEasy FS PCR-Free DNA Library Prep Set

MGIEasy FS PCR-Free DNA Library Prep Set is specifically designed for constructing WGS libraries without PCR amplification for the MGI high-throughput sequencing platform. For the high-quality and low-bias fragmentation enzyme, the set could fragment 50-1000 ng gDNA. The distribution of fragmented DNA is similar to that fragmented by mechanical techniques. The set combined with the automatic sample preparation system MGISP, could provide an automatic library preparation workflow. FS PCR-free combined with MGI DNBSEQ^TM sequencer, is truly PCR-free sequencing with no amplification errors accumulation.

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MGIEasy FS PCR-Free DNA Library Prep Set （96 RXN）

Item No.：1000013455

Library and Circularization	MGIEasy FS PCR-Free DNA Library Prep Kit	1000013459
Adapter	MGIEasy PF Adapters-96 (plate) Kit	1000013461
Beads	MGIEasy DNA Clean Beads (50 mL)	940-001594-00

MGIEasy FS PCR-Free DNA Library Prep Set （16 RXN）

Item No.：1000013454

Library and Circularization	MGIEasy FS PCR-Free DNA Library Prep Kit	1000013458
Adapter	MGIEasy PF Adapters-16 (tube) Kit	1000013460
Beads	MGIEasy DNA Clean Beads (8 mL)	940-001596-00

Features

Good compatibility for different species fragmentation Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the consistent and concentrated fragment range.	Stable library yield The modified reaction system increases the library quality and results in a stable library yield.
Simple and automated workflow Library construction can be completed within 3.9 hours. Compatible with automatic sample preparation systems to provide an automated solution.	No amplification error accumulation WGS PCR-free library preparation in combination with sequencing by the MGI DNBSEQ^TM platform has no amplification error accumulation, resulting in better genome fidelity.
Good coverage uniformity The genome coverage of FS PCR-free achieved comparable performance to Covaris PCR-free. The FS PCR-free technique covers the GC-rich, promoter and repetitive regions. Significantly better than traditional WGS methods.	Excellent variant performance The variant detection performance of FS PCR-free is similar to Covaris PCR-free, and better than traditional PCR, especially InDels.

Good compatibility for different species fragmentation

Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the consistent and concentrated fragment range.

Stable library yield

The modified reaction system increases the library quality and results in a stable library yield.

Simple and automated workflow

Library construction can be completed within 3.9 hours. Compatible with automatic sample preparation systems to provide an automated solution.

No amplification error accumulation

WGS PCR-free library preparation in combination with sequencing by the MGI DNBSEQ^TM platform has no amplification error accumulation, resulting in better genome fidelity.

Good coverage uniformity

The genome coverage of FS PCR-free achieved comparable performance to Covaris PCR-free. The FS PCR-free technique covers the GC-rich, promoter and repetitive regions. Significantly better than traditional WGS methods.

Excellent variant performance

The variant detection performance of FS PCR-free is similar to Covaris PCR-free, and better than traditional PCR, especially InDels.

Product Specification

Product Name	MGIEasy FS PCR-Free DNA Library Prep Set
Application	Whole genome sequencing
The version of Set	V1.2 (16RXN), V1.3 (96RXN)
Configuration (Cat.)	16 RXN(1000013454), 96 RXN(1000013455)
Validity period	9 months
Assay Time	~3.9 hrs
Input Quantity	50-1000 ng gDNA
Sample types	gDNA, WGA DNA
Species Compatibility	Human, animals, plants, fungi, bacteria, metagenomics, etc.
Fragmentation Method	Enzymatic
Recommended Insert Size	350-660 bp
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50,DNBSEQ-T7
Recommended Read Length	SE100, PE100, PE150

Recommended MGI Process

Application	Library Preparation	Sequening	Analysis
Human WGS sequencing (High depth)	MGIEasy FS PCR-Free DNA Library Prep Set MGISP-100 MGISP-960	DNBSEQ-G400* DNBSEQ-G50* DNBSEQ-T7*	MegaBOLT Bioinformatics analysis accelerator -WGS germline mutation analysis software -WGS somatic mutation analysis software

Application