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MGIEasy Pa-SNPs Genotyping Kit

MGIEasy Pa-SNPs Genotyping Kit is developed for rapid detection of 2010 high-frequency single nucleotide polymorphisms (SNPs) of human. The kit is designed on MGI's proprietary ATOPlex platform – a multiplex PCR design platform. It includes all the required reagents to prepare sequencing libraries from DNA samples. MGIEasy Pa-SNPs Genotyping Kit can be applied to various sample types, such as cfDNA from plasma, gDNA from blood, saliva, tissue or sperm. It is specifically designed for use in forensics genomics applications, including non-invasive prenatal paternity testing, paternity testing and individual identification



MGIEasy Pa-SNPs Genotyping Kit (96 RXN)

Item No.:1000016270
Box 1 Including PCR primer, PCR enzyme, buffer, circularization reagents, digestion reagents and anti-contamination reagents
Box 2 Including 96 PCR barcode primer
Box 3 Two types of beads for PCR product cleanup and digestion product cleanup
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Features

Easy to use
Easy to use

Two-step PCR in one tube to amplify 2010 SNPs;

Fast and efficient workflow
Fast and efficient workflow

6.5 hours in total with only 1.5 hours hands-on time;

High sensitivity
High sensitivity
Sample input as little as 1 ng of DNA;
High accuracy
High accuracy
Accurate detection of 2% minor contributors;
Wide range of applications
Wide range of applications
Specifically designed for cfDNA and highly degraded samples.
Easy to use
Easy to use

Two-step PCR in one tube to amplify 2010 SNPs;

Fast and efficient workflow
Fast and efficient workflow

6.5 hours in total with only 1.5 hours hands-on time;

High sensitivity
High sensitivity
Sample input as little as 1 ng of DNA;
High accuracy
High accuracy
Accurate detection of 2% minor contributors;
Wide range of applications
Wide range of applications
Specifically designed for cfDNA and highly degraded samples.

Product Specification

Product name

MGIEasy Pa-SNPs Genotyping Kit

Version V1.0

Reaction/kit

96 RXN/kit

Catalog No.

1000016270

Species Compatibility

Human

Sample types

cell free DNA (cfDNA) from plasma and genomic DNA (gDNA) from blood, tissues, blood spot, saliva, buccal swabs, semen, hair, and nails

Recommend Input

Quantity

cfDNA, 8-20 ng cfDNA, DNA concentration ≥0.4 ng/µL; gDNA, 1-20 ng;


Library Method

Multiplex PCR

Amplicon Size

60-90 bp

Library Time
6.5 h

Recommended Read Length

SE50

Platform

 Compatibility

DNBSEQ-G400, DNBSEQ-G50,  BGISEQ-500

Recommended sequencing

data

cfDNA, ≥25M reads; gDNA, ≥ 5M reads

Recommended MGI Process

Applications
Library preparation
Sequencing
Bioinformation analysis
  • Non-invasive prenatal paternity testing

  • MGIEasy Pa-SNPs Genotyping Kit
  • DNBSEQ-G400(SE50)

  • DNBSEQ-G50(SE50)

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