MGI gene sequencing company

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ATOPlex Platform
ATOPlex Platform


ATOPlex is a massively parallel sequencing (MPS) solution that applies MGI proprietary ultra-high multiplex PCR-based enrichment technique in customized panel design. It covers DNA, RNA and DNA methylation sequencing in multiple fields such as medicine, forensic, agriculture, DTC and etc. MGI provides targeted sequencing total solution including product design, library construction kits, automated workflow, high-throughput sequencers and etc.


ATOPlex Platform
ATOPlex Platform
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Design
Design
Process
Process
Sequencer
Sequencer
Workflow of ATOPlex


ATOPlex is a massively parallel sequencing (MPS) solution that applies MGI proprietary ultra-high multiplex PCR-based enrichment technique in customized panel design. It covers DNA, RNA and DNA methylation sequencing in multiple fields such as medicine, forensic, agriculture, DTC and etc. MGI provides targeted sequencing total solution including product design, library construction kits, automated workflow, high-throughput sequencers and etc.


SampleSample PCRPCR Pre-SequencePre-Sequence SequencingSequencing AnalysisAnalysis
0.5hours 2.5hours 2hours 12-72hours 2-6hours
Lysis
Lysis
PCR
1stPCR 2ndPCR
Pre-Sequence
ssCir DNB
Sequencing
Run sequence
Analyze data
Analyze data
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ATOPlex Platform
Auto-workflow
Automated design workflow
Auto-workflow
Automated design workflow
Trace-samples
diverse trace samples
Trace-samples
diverse trace samples
One-tube
amplified in one tube
One-tube
amplified in one tube
Pure-PCR
efficiently eradicate aerosol amplicons
Pure-PCR
efficiently eradicate aerosol amplicons
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Note::

    Auto-workflow: Automated primer design workflow; automated library preparation workflow.

    Trace-samples: Library preparation from diverse trace samples with as little as 0.1 ng of input DNA;Library could be prepared directly from various crude lysates without DNA extraction, which significantly reduces hands-on time.

    One-tube: As many as 10000 amplicons, including successive DNA regions, could be amplified in one tube;Simple one-tube, 2-step protocol that minimizes sample loss and contamination.

    Pure-PCR: The clean system in the amplificationreaction could efficiently eradicate aerosol amplicons and minimize the risk of cross contamination. The amplification system could tolerate multiple PCR inhibitors present in the input DNA, which minimizes preparation failure rate.

Workflow of ATOPlex customized panel
Workflow of ATOPlex customized panel
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Available Design Options
Size Type Process Species Parameter Kit
Region Mutation Time Sample Performance Reagent
  • 1 kb~1 m
  • 10~10000 plex
  • 50~300bp
  • snv
  • Indel
  • 5.0 h Total time
  • 1.5 h hands-on time
  • DNA
  • saliva
  • blood
  • spot
  • hair
  • FFPE
  • tissue
  • >95%
    Map rate
  • >95%
    On target
  • >90%
    0.2XMean depth
  • PCR reagent
  • Primer pool
  • Barcode primer
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