Based on MGI's self-developed multiplex PCR technology, ATOPlex platform can design specific multiplex PCR primers for targeted genomes, which applies to tumor, pathogen, forensic, and so on, specifically amplifying targeted regions and preparing the NGS library, detecting SNV/Indel/CNVs.
Compared with other multiplex PCR methods, ATOPlex multiplex PCR can effectively reduce the formation of primers dimer and achieve the amplification of targeted gene fragments in one tube, thus minimizing the risk of sample cross-contamination. The technique can accurately quantify sample concentration through the added artificial DNA, while the process is easy to operate with two PCR steps, including 4 individual operations: gene-specific PCR, beads clean up, universal PCR, and beads clean up again.
*Unless otherwise informed, all sequencers and sequencing reagents are not available in Germany, USA, Spain, UK, Hong Kong, Sweden, Belgium and Italy.
Auto-workflow: Automated primer design workflow; automated library preparation workflow.
Trace-samples: Library preparation from diverse trace samples with as little as 0.1 ng of input DNA; Library could be prepared directly from various crude lysates without DNA extraction, which significantly reduces hands-on time.
One-tube: As many as 100,00 amplicons, including successive DNA regions, could be amplified in one tube; Simple one-tube, 2-step protocol that minimizes sample loss and contamination.
Pure-PCR: The clean system in the amplificationreaction could efficiently eradicate aerosol amplicons and minimize the risk of cross contamination. The amplification system could tolerate multiple PCR inhibitors present in the input DNA, which minimizes preparation failure rate.