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MGIEasy RNA Library Prep Set

MGIEasy RNA Library Prep Set provides an efficient package for generating libraries from a wide range (10 ng-1 ug) of total RNA that is suitable for MGI high-throughput sequencing platforms. It is easy to use and can be widely applied to human, animal, plant and microbe transcriptome research for comprehensive and rapid analysis of gene expression changes, examining rare and novel transcripts, discovery of alternate splicing events, gene fusions, SNPs and allele-specific expression

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MGIEasy RNA Library Prep Set（16 RXN）

Item No.：1000006383

Library	MGIEasy RNA Library Prep Kit V3.0（16 RXN）	1000005274
Adapters	MGIEasy DNA Adapters-16(Tubes) Kit V1.0（16 RXN, 16 barcodes）	1000005284
Clean Beads	MGIEasy DNA Clean Beads	1000005278
Circularization	MGIEasy Circularization Module V2.0	1000005260

MGIEasy RNA Library Prep Set（96 RXN）

Item No.：1000006384

Library	MGIEasy RNA Library Prep Kit V3.0（96 RXN）	1000005276
Adapters	MGIEasy DNA Adapters-96(Plate) Kit V1.0（96 RXN, 96 barcodes）	1000005282
Clean Beads	MGIEasy DNA Clean Beads	1000005279
Circularization	MGIEasy Circularization Module V2.0	1000005260

Features

Wide range of total RNA input Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.	Compatible with various sample types Including low-quality RNA, FFPE RNA and plasma samples.
Excellent coverage Accurate and comprehensive mapping of transcripts.	High uniformity Enables detection of complete transcripts without bias at either end.
Easy to Use Simple protocol and short operation time, suitable for automated library preparation instrument.	Flexibility Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.

Wide range of total RNA input

Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.

Compatible with various sample types

Including low-quality RNA, FFPE RNA and plasma samples.

Excellent coverage

Accurate and comprehensive mapping of transcripts.

High uniformity

Enables detection of complete transcripts without bias at either end.

Easy to Use

Simple protocol and short operation time, suitable for automated library preparation instrument.

Flexibility

Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.

Recommended Applications

Sample type	RNA enrichment method	Read length	Applications
Eukaryotic total RNA with high integrity	Poly(A)+ mRNA enriched by oligo(dT) beads	SE50/PE100/PE150	mRNA depleted with mRNA depletion kit
mRNA quantification and transcriptome analysis	Prokaryotic total RNA	SE50/PE100/PE150	rRNA depleted with rRNA depletion kit
RNA quantification and transcriptome analysis	Degraded RNA from FFPE samples or plasma cell-free RNA etc.	SE50/PE100	rRNA depleted with rRNA depletion kit

Product Specification

Product name	MGIEasy RNA Library Prep Set
Version	V3.0
Reaction/kit	16 RXN （1000006383） / 96 RXN （1000006384）
Storage temperature requirement	-18℃~-25℃
Input Quantity	10 NG -1 UG TOTAL RNA
Sample types	Tissues and FFPE sample
Species Compatibility	Human, animals, plants, fungi and bacteria such as, mouse, rice, Arabidopsis, yeast and E.coli.
Assay Time	7 hrs
Applications	RNA-Seq, Transcriptome Sequencing
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50
Recommended Read Length	SE50/PE100/PE150
Recommended sequencing data per sample	25 M raw reads（SE50）/8 Gb raw data（PE100/PE150）

Recommended MGI Process

Applications	Library preparation	Sequencing	Bioinformation analysis
RNA-SEQ	MGISP-100 MGISP-960 MGIEasy RNA Library Prep Set	DNBSEQ-G400（SE50） DNBSEQ-50（SE50）
Transcriptome Sequencing	MGISP-100 MGISP-960 MGIEasy RNA Library Prep Set	DNBSEQ-G400（PE100/ PE150） DNBSEQ-G50（PE100/ PE150）