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MGIEasy RNA Library Prep Set

MGIEasy RNA Library Prep Set provides an efficient solution for generating libraries from a wide range (10 ng-1 ug) of total RNA that is suitable for MGI high-throughput sequencing platforms. It is easy to use and can be widely applied to human, animal, plant and microbe transcriptome research for comprehensive and rapid analysis of gene expression changes, examining rare and novel transcripts, discovery of alternate splicing events, gene fusions, SNPs and allele-specific expression


MGIEasy RNA Library Prep Set(16 RXN)

Item No.:1000006383
Library MGIEasy RNA Library Prep Kit V3.0(16 RXN) 1000005274
Adapters MGIEasy DNA Adapters-16(Tubes) Kit V1.0(16 RXN, 16 barcodes) 1000005284
Clean Beads MGIEasy DNA Clean Beads 1000005278
Circularization MGIEasy Circularization Module V2.0 1000005260
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MGIEasy RNA Library Prep Set(96 RXN)

Item No.:1000006384
Library MGIEasy RNA Library Prep Kit V3.0(96 RXN) 1000005276
Adapters MGIEasy DNA Adapters-96(Plate) Kit V1.0(96 RXN, 96 barcodes) 1000005282
Clean Beads MGIEasy DNA Clean Beads 1000005279
Circularization MGIEasy Circularization Module V2.0 1000005260
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Features

Wide range of total RNA input
Wide range of total RNA input
Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.
Compatible with various sample types
Compatible with various sample types

Including low-quality RNA, FFPE RNA and plasma samples.

Excellent coverage
Excellent coverage

Accurate and comprehensive mapping of transcripts.

High uniformity
High uniformity
Enables detection of complete transcripts without bias at either end.
Easy to Use
Easy to Use

Simple protocol and short operation time, suitable for automated library preparation instrument.

Flexible Solutions
Flexible Solutions

Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.

Wide range of total RNA input
Wide range of total RNA input
Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.
Compatible with various sample types
Compatible with various sample types

Including low-quality RNA, FFPE RNA and plasma samples.

Excellent coverage
Excellent coverage

Accurate and comprehensive mapping of transcripts.

High uniformity
High uniformity
Enables detection of complete transcripts without bias at either end.
Easy to Use
Easy to Use

Simple protocol and short operation time, suitable for automated library preparation instrument.

Flexible Solutions
Flexible Solutions

Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.

Recommended Applications

Sample type RNA enrichment method Read length Applications
Eukaryotic total RNA with high integrity   Poly(A)+ mRNA enriched by oligo(dT) beads SE50/PE100/PE150 SE50/ PE100/ PE150
mRNA quantification and transcriptome analysis Prokaryotic total RNA SE50/PE100/PE150 rRNA depleted with rRNA depletion kit
RNA quantification and transcriptome analysis Degraded RNA from FFPE samples or plasma cell-free RNA etc. SE50/PE100 rRNA depleted with rRNA depletion kit
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Product Specification

Product name

MGIEasy RNA Library Prep Set

Version V3.0
Reaction/kit 16 RXN (1000006383) / 96 RXN (1000006384)

Storage temperature requirement

-18℃~-25℃

Input Quantity

10 NG -1 UG TOTAL RNA

Sample types

Tissues and FFPE sample

Species Compatibility

Human,animals, plants, fungi and bacteria such as, mouse, rice, Arabidopsis, yeast and E.coli.

Assay Time

7 h
Applications

RNA-Seq, Transcriptome Sequencing

Platform Compatibility

BGISEQ-500、MGISEQ-2000、MGISEQ-200、DNBSEQ-G400、DNBSEQ-G50

Recommended Read Length

SE50/PE100/PE150

Recommended sequencing data per sample

25 M raw reads(SE50)/8 Gb raw data(PE100/PE150)

Recommended MGI Process

Applications
Library preparation 
Sequencing 
Bioinformation analysis
  • RNA-SEQ

  • Transcriptome Sequencing

  • MGISEQ-2000 / DNBSEQ-G400(PE100/ PE150)

  • MGISEQ-200 / DNBSEQ-G50(PE100/ PE150)

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