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MGIEasy stLFR Library Prep Kit*

MGIEasy stLFR Library Prep Kit* is the world’s first partition-less long fragment DNA co-barcoding library prep kit [1]. Based on the principle of DNA co-barcoding, which adds the same barcode sequence to sub-fragments of the original long DNA molecule, stLFR achieves long range genetic information using high throughput and highly precise short read sequencing. Coupled with the MGI DNBSEQ^TM sequencing platform, stLFR enables high quality small variants calling, phasing of over 99% of the human genome, better genome coverage, detection of large structural variants, and other long read applications.

[1] - Wang et al, 2019. Genome Res.29: 798-808.

* This Product is Only for Sale in the United States, and for Research Use Only.

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MGIEasy stLFR Library Prep Kit (16 rxn)

Item No.：940-000193-00

MGIEasy stLFR Library Prep Kit (48rxn)

Item No.：940-000194-00

Features

√Long range information Long fragment read lengths of 50-70kb on average, up to 300kb	√Ultra-low DNA input Only 1.5ng required, 98% less than single molecule sequencing methods
√Haplotype phasing Derive heterozygous variants linkage information for genetic disease and reproductive health applications	√Single-tube reaction No need for nanoliter liquid handling or additional purchase of microfluidic systems
√Automatable library prep Automatable library prep on the MGISP-960, minimal hands-on time and effort	√Structural variants detection Detect copy number variants, deletions, inversions, and balanced translocations.

√Long range information

Long fragment read lengths of 50-70kb on average, up to 300kb

√Ultra-low DNA input

Only 1.5ng required, 98% less than single molecule sequencing methods

√Haplotype phasing

Derive heterozygous variants linkage information for genetic disease and reproductive health applications

√Single-tube reaction

No need for nanoliter liquid handling or additional purchase of microfluidic systems

√Automatable library prep

Automatable library prep on the MGISP-960, minimal hands-on time and effort

√Structural variants detection

Detect copy number variants, deletions, inversions, and balanced translocations.

Product Specification

Product Name	MGIEasy stLFR Library Prep Kit*
Specification(Product No.)	16 RXN (940-000193-00), 48 RXN(940-000194-00)
Shelf life	12 months
Input material	Genomic DNA
Species supported	Multiple species
Type of samples	High molecular weight DNA
Requirements for DNA sample	Sample concentration should be more than 1 ng/μL, the A260/A280 value of the DNA sample should be 1.6 to 2.2. The mean length of the initial DNA fragments should be longer than 40 Kb with no degradation below 40 Kb. DNA fragments with longer length will produce better results.
Library prep process	Enzymatic shearing, co-barcoding of long DNA fragments by bead capture and ligation, PCR amplification, circularization, and make DNB.
Compatible sequencing platforms	DNBSEQ-G400, DNBSEQ-T7
Sequencing read length	PE100+42+10
Recommended coverage/ number of raw reads	For human resequencing purpose (30x): 130Gb or 650M PE100 raw reads

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