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MGIEasy stLFR Library Prep Kit

MGIEasy stLFR Library Prep Kit is the world's first partition-less long fragment DNA co-barcoding library prep kit. Based on the patented DNA co-barcoding technology, that is adding the same barcode sequence to sub-fragments of the original long DNA molecule, stLFR could read the long range genetic information very accurately. With MGI's DNBSEQTM the world's most accurate sequencing technology, stLFR enables high quality small variants calling, phasing diploid genomes, detection of structure variations and other long read applications.


MGIEasy stLFR Library Prep Kit (16 rxn)

Item No.:1000005622
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Features

Long range information
Long range information
Long range information with accurate short-read sequencing.
No pre-amplification
No pre-amplification
High quality WGS libraries from only 1.5 ng DNA.
Powerful detection of structure variations
Powerful detection of structure variations
Over 10 Mb of Haplotype Contig N50 and powerful detection of structure variations, such as deletions, inversions, translocations and insertions.
​One tube reaction
​One tube reaction
No need for nanoliter liquid handling or microfluidic systems.
Easily automatable solution
Easily automatable solution
Magnetic beads based, easily automatable solution.
Long range information
Long range information
Long range information with accurate short-read sequencing.
No pre-amplification
No pre-amplification
High quality WGS libraries from only 1.5 ng DNA.
Powerful detection of structure variations
Powerful detection of structure variations
Over 10 Mb of Haplotype Contig N50 and powerful detection of structure variations, such as deletions, inversions, translocations and insertions.
​One tube reaction
​One tube reaction
No need for nanoliter liquid handling or microfluidic systems.
Easily automatable solution
Easily automatable solution
Magnetic beads based, easily automatable solution.

Product Specification

Product Name

MGIEasy stLFR Library Prep Kit

PN. 1000005622
Specification

16 RXN

Shelf life
6 months
Input material

Genome DNA

Species supported
Multiple species
Type of samples
High molecular weight DNA
Requirements for DNA sample

The DNA required for stLFR library needs to be as large as it can be. The larger the DNA is the better the performance will be. Usually the DNA isolated with methods suggested below will be with a main band greater than 100 kb when analyzing with pulse-field electrophoresis. The suggested DNA isolated kits are Qiagen MagAttract HMW DNA Kit (Cat No./ID: 67563) or Agilent Technologies RECOVEREASE DNA ISOLATION KIT (Catalog No.50-126-011) The required concentration of DNA should be more than1 ng/ul and the total amount should be more than 500ng.

Library process

Shear DNA with enzyme, perform DNA co-barcoding, introduce another adapter, amplify with PCR and make DNB

Sequencing platforms suggested

BGISEQ-500, DNBSEQ-G400, MGISEQ-2000 

Sequencing read length suggested

PE100+42

Suggested coverage/Raw reads number

For human resequencing purpose: 30X, ~650M raw reads (PE100)

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