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MGIEasy stLFR Library Prep Kit

MGIEasy stLFR Library Prep Kit is the world’s first partition-less long fragment DNA co-barcoding library prep kit [1]. Based on the principle of DNA co-barcoding, which adds the same barcode sequence to sub-fragments of the original long DNA molecule, stLFR achieves long range genetic information using economical and highly precise short read sequencing. Coupled with the MGI DNBSEQTM sequencing platform, stLFR enables high quality small variants calling, phasing of over 99% of the human genome, detection of structure variations, de novo assembly, and other long read applications.

[1] - Wang et al, 2019. Genome Res 29: 798-808.

MGIEasy stLFR Library Prep Kit

MGIEasy stLFR Library Prep Kit (16 rxn)

Item No.:940-000193-00

MGIEasy stLFR Library Prep Kit (48rxn)

Item No.:940-000194-00

Product Specification

Product Name

MGIEasy stLFR Library Prep Kit

Specification(Product No.)

16 RXN (940-000193-00), 48 RXN(940-000194-00)

Shelf life
12 months
Input material

Genomic DNA

Species supported
Multiple species
Type of samples
High molecular weight DNA
Requirements for DNA sample

Sample concentration should be more than 1 ng/μL, the A260/A280 value of the DNA sample should be 1.6 to 2.2. The mean length of the initial DNA fragments should be longer than 40 Kb with no degradation below 40 Kb. DNA fragments with longer length will produce better results.

Library prep process

Enzymatic shearing, co-barcoding of long DNA fragments by bead capture and ligation, PCR amplification, circularization, and make DNB.

Compatible sequencing platforms


Sequencing read length


Recommended coverage/ number of raw reads

For human resequencing purpose (30xdepth): 130Gb or 650M PE100 raw reads

*Remark: *Unless otherwise informed, StandardMPS and CoolMPS sequencing reagents, and sequencers for use with such reagents are not available in Germany, USA, Spain, UK, Hong Kong, Sweden, Belgium, Italy, Finland, Czech Republic, Switzerland and Portugal.

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