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MGIEasy Whole Genome Bisulfite Sequencing Library Prep Kit

DNA methylation is an important epigenetic mechanism that cells used to control the temporal and spatial expression of genes. MGIEasy Whole Genome Bisulfite Sequencing Library Prep Kit provides an efficient and accurate method for genome-wide methylation library construction and sequencing. It can analyze the DNA methylation status at single base level, and thus enable the construction of a fine genome-wide methylation map. The kit prepares libraries compatible with MGI sequencing platforms from 10-100ng fragmented DNA.

Kit components:



MGIEasy Whole Genome Bisulfite Sequencing Library Prep Kit (16 RXN)

Item No.:1000005251
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Features

Extremely low DNA input amount
Extremely low DNA input amount
Requires only 10-100ng fragmented DNA.
Compatible with various sample types
Compatible with various sample types
Compatible with samples from animals, plants, fungi, and etc. The sample types include but are not limited to gDNA, FFPE and blood samples.
Easy to operate
Easy to operate
The library preparation steps are easy to operate and only require 8 hours.
Superior data performances
Superior data performances
High data utilization rate, high genome coverage and accurate methylation detection.
Extremely low DNA input amount
Extremely low DNA input amount
Requires only 10-100ng fragmented DNA.
Compatible with various sample types
Compatible with various sample types
Compatible with samples from animals, plants, fungi, and etc. The sample types include but are not limited to gDNA, FFPE and blood samples.
Easy to operate
Easy to operate
The library preparation steps are easy to operate and only require 8 hours.
Superior data performances
Superior data performances
High data utilization rate, high genome coverage and accurate methylation detection.

Product Specification

Product name

MGIEasy Whole Genome Bisulfite Sequencing Library Prep Kit

Version V2.0

Packaging (Part number)

16 RXN (1000005251)

Storage

-25℃~-18℃(Refer to kit label)

Required sample input

Fragmented DNA,input DNA 10-100 ng, concentration > 0.3 ng/μL

Sample types

Tissues, plasma, FFPE samples

Compatible species

Animals, plants, fungi

Read length

PE100/PE150

Fragmentation method

Covaris (no fragmentation for cfDNA)

Library fragment size

100 -500 bp

Compatible sequencing platforms

BGISEQ-500RS, DNBSEQ-G400RS, MGISEQ-2000RS

Recommended MGI Process

Applications
Library preparation
Sequencing
Bioinformation analysis
  • Whole Genome Bisulfite Sequencing

  • MGIEasy Whole Genome Bisulfite Sequencing Library Prep Kit

  • BGISEQ-500RS, G400RS, MGISEQ-2000RS(PE100、PE150)

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