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MGIEasy RNA Directional Library Prep Set

MGIEasy RNA Directional Library Prep Set provides an efficient solution for generating libraries suitable for MGI high-throughput sequencing platforms from total RNA. It can be used to identify RNA stranded information that from which of the two DNA strands a given RNA transcript originates. Identifying strand origin increases the percentage of reads that align to the transcriptome and provides more accurate information for studies in regulation of gene expression and gene functional analysis. Maintaining strand orientation also allows identification of antisense expression and novel genes.

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MGIEasy RNA Directional Library Prep Set（96 RXN）

Item No.：1000006386

Library	MGIEasy RNA Directional Library Prep Kit V2.0（96 RXN）	1000005272
Adapters	MGIEasy DNA Adapters-96(Plate) Kit V1.0（96 RXN, 96 barcodes）	1000005282
Clean Beads	MGIEasy DNA Clean Beads	1000005279
Circularization	MGIEasy Circularization Module V2.0	1000005260

MGIEasy RNA Directional Library Prep Set（16 RXN）

Item No.：1000006385

Library	MGIEasy RNA Directional Library Prep Kit V2.0（16 RXN）	1000005270
Adapters	MGIEasy DNA Adapters-16(Tubes) Kit V1.0（16 RXN, 16 barcodes）	1000005284
Clean Beads	MGIEasy DNA Clean Beads	1000005278
Circularization	MGIEasy Circularization Module V2.0	1000005260

Features

Wide range of total RNA input Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.	Compatible with various sample types Including low-quality RNA, FFPE RNA and plasma samples.
Excellent coverage quality Accurate and comprehensive mapping of transcripts.	High uniformity Enables high uniform from transcripts 5 'to 3' ends and superior 3’end coverage.
Accurate detection of strand orientation Identify transcript origin, detect antisense transcripts, enhances transcript annotation.	Easy to Use Simple protocol and short operation time, suitable for automated library preparation instrument.
Flexibility Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.

Wide range of total RNA input

Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.

Compatible with various sample types

Including low-quality RNA, FFPE RNA and plasma samples.

Excellent coverage quality

Accurate and comprehensive mapping of transcripts.

High uniformity

Enables high uniform from transcripts 5 'to 3' ends and superior 3’end coverage.

Accurate detection of strand orientation

Identify transcript origin, detect antisense transcripts, enhances transcript annotation.

Easy to Use

Simple protocol and short operation time, suitable for automated library preparation instrument.

Flexibility

Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.

Recommended Applications

Sample type	RNA enrichment method	Read length	Applications
Eukaryotic total RNA with high integrity	Poly(A)+ mRNA enriched by oligo(dT) beads	SE50/PE100/PE150	mRNA quantification and transcriptome analysis
Eukaryotic total RNA with high integrity	rRNA depleted with rRNA depletion kit	PE100/PE150	lncRNA and transcriptome analysis
Prokaryotic total RNA	rRNA depleted with rRNA depletion kit	PE100/PE150	RNA quantification and transcriptome analysis
Degraded RNA from FFPE samples or plasma cell-free RNA etc.	rRNA depleted with rRNA depletion kit	SE50/PE100	RNA quantification and transcriptome analysis

Product Specification

Product name	MGIEasy RNA Directional Library Prep Set
Version	V2.0
Reaction/kit	16 RXN （1000006385）,96 RXN （1000006386）
Storage temperature requirement	-18℃~-25℃
Input Quantity	10 ng-1 ug total RNA
Sample types	Tissues and FFPE sample
Species Compatibility	Human,animals, plants, fungi and bacteria such as, mouse, rice, Arabidopsis, yeast and E.coli.
Assay Time	7 hrs
Applications	RNA-Seq, Transcriptome Sequencing, total RNA sequencing, lncRNA Sequencing
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50
Recommended Read Length	SE50/PE100/PE150
Recommended sequencing data per sample	25 M raw reads（SE50）/ 8 Gb raw data（PE100/PE150）

Recommended MGI Process

Applications	Library preparation	Sequencing	Bioinformation analysis
Total RNA Sequencing	MGISP-100 MGISP-960 MGIEasy RNA Directional Library Prep Set + MGIEasy rRNA Depletion Kit	DNBSEQ-G400（PE100/ PE150） DNBSEQ-G50（PE100/ PE150）
lncRNA Sequencing		DNBSEQ-G400（PE100/ PE150） DNBSEQ-G50（PE100/ PE150）