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MGIEasy Fast Hybridization and Wash Kit

MGIEasy Fast Hybridization and Wash Kit is designed for targeted capture with rapid hybridization elution steps of DNA probes and includes hybridization elution reagents and streptavidin magnetic beads. It has broad compatibility, standardizing the hybridization process for DNA probes, and reduces hybridization time to within 2 hours while ensuring data quality, significantly shortening the liquid-phase probe hybridization capture time. By flexibly pairing with the MGIEasy series of library preparation kits, it can be used for the construction and targeted enrichment of exome libraries and panel capture libraries on the MGI high-throughput sequencing platform.

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MGIEasy Fast Hybridization and Wash Kit (96 RXN)

Item No.：940-001973-00

Component kits	Product name of component kit	Part No. of component kit	Quantity of component kit in a Set
Hybridization elution reagents	MGIEasy Fast Hybridization and Wash Kit (BOX 1)	940-001826-00	1
Streptavidin magnetic beads	MGIEasy Fast Hybridization and Wash Kit (BOX 2)	940-001828-00	1

MGIEasy Fast Hybridization and Wash Kit (16 RXN)

Item No.：940-001974-00

Component kits	Product name of component kit	Part No. of component kit	Quantity of component kit in a set
Hybridization elution reagents	MGIEasy Fast Hybridization and Wash Kit (BOX 1)	940-001825-00	1
Streptavidin magnetic beads	MGIEasy Fast Hybridization and Wash Kit (BOX 2)	940-001823-00	1

Features

Fast hybridization Reduce hybridization time to within 2 hours while ensuring data quality, significantly shortening the liquid-phase probe hybridization capture time. Combined with MGIEasy Fast FS Library Prep Set V2.0, it can achieve to complete the prep from gDNA to enriched libraries within one day.	Flexible hybridization Support flexible selection between 0.5 h-2 h fast hybridization and traditional overnight hybridization.
Broad compatibility Compatible with multiple DNA probes (such as IDT, Twist, Nanod, BOKE).	Enhanced performance Better post-capture yield, low duplication rate, high capture rate, deep and uniform coverage.
Easy to use Simplify the operation to minimize the possibility of human errors. Automation compatible.

Fast hybridization

Reduce hybridization time to within 2 hours while ensuring data quality, significantly shortening the liquid-phase probe hybridization capture time. Combined with MGIEasy Fast FS Library Prep Set V2.0, it can achieve to complete the prep from gDNA to enriched libraries within one day.

Flexible hybridization

Support flexible selection between 0.5 h-2 h fast hybridization and traditional overnight hybridization.

Broad compatibility

Compatible with multiple DNA probes (such as IDT, Twist, Nanod, BOKE).

Enhanced performance

Better post-capture yield, low duplication rate, high capture rate, deep and uniform coverage.

Easy to use

Simplify the operation to minimize the possibility of human errors. Automation compatible.

Recommended Applications

Probes and Hybridization reagent for capture	Per-capture library preparation	Sequencing	Application
Probes: commercial DNA probes Hybridization: MGIEasy Fast Hybridization and Wash Kit Blocker: MGIEasy Dual Barcode Exome Capture Accessory Kit	MGIEasy Fast FS Library Prep Set V2.0 & MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400（PE100/PE150） or DNBSEQ-G99 (PE150) or DNBSEQ-T7 (PE100/PE150)	Whole exome sequencing/ target sequencing Whole exome sequencing/target sequencing target sequencing
MGIEasy UDB Universal Library Prep Set
MGIEasy Duplex UMI Universal Library Prep Set

Probes and Hybridization reagent for capture

Per-capture library preparation

Sequencing

Application

Probes: commercial DNA probes

Hybridization: MGIEasy Fast Hybridization and Wash Kit

Blocker: MGIEasy Dual Barcode Exome Capture Accessory Kit

MGIEasy Fast FS Library Prep Set V2.0

& MGIEasy Dual Barcode Circularization Kit

DNBSEQ-G400（PE100/PE150）

DNBSEQ-G99 (PE150)

DNBSEQ-T7 (PE100/PE150)

Whole exome sequencing/ target sequencing

Whole exome sequencing/target sequencing

target sequencing

MGIEasy UDB Universal Library Prep Set

MGIEasy Duplex UMI Universal Library Prep Set

Product Specification

Applications	Hybridization capture of target regions in human samples, such as WES, small panel sequencing
Probes	commercial DNA probes (such as IDT, Twist, Nanod, BOKE)
Hybridization time	0.5h~24h
Sample type	Huamn gDNA, FFPE DNA, cfDNA
Platform Compatibility	MGISEQ-2000, DNBSEQ-G99, DNBSEQ-T7
Pre-library	pre-PCR library prepared using MGI double barcode adapters for hybridization reaction. (It is recommended to frag the sample to be constructed into fragments with a main banddistribution of 200~300bp）
multi-plex hybridization	Compatible with single-plex and multi-plex samples hybridization (supports multi-hybridization of up to 12 samples)
Circularization	MGI universal circularization

Recommended MGI Process

Applications	Library preparation	Sequencing	Data analysis
Human WES	MGIEasy Fast FS Library Prep Set V2.0 MGIEasy Fast Hybridization and Wash Kit MGIEasy Dual Barcode Exome Capture Accessory Kit Other commercialized WES DNA probes MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-G99 DNBSEQ-T7	MegaBOLT Bioinformatics analysis accelerator -WES Germline variant calling pipeline -WES Somatic variant calling pipeline
Capture panel sequencing	MGIEasy Fast FS Library Prep Set V2.0 MGIEasy Fast Hybridization and Wash Kit MGIEasy Dual Barcode Exome Capture Accessory Kit Other commercialized DNA probes MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-G99 DNBSEQ-T7

Related Products

MGIEasy UDB Universal Library Prep Set

Applications: Whole genome sequencing

Platform: DNBSEQ-T7, DNBSEQ-G400, DNBSEQ-G50

Reaction/KIT: 16 RXN/96 RXN/192RXN

MGIEasy Fast FS Library Prep Set V2.0

Application: Human WGS, Plants & Animals WGS, Microbial WGS, Metagenomics sequencing, WES

Platform Compatibility: DNBSE-G400, DNBSEQ-G50, DNBSEQ-G99, DNBSEQ-E25, DNBSEQ-T7, DNBSEQ-T10×4, DNBSEQ-T20×2

Reaction/kit: 16 RNX,96 RNX,192 RXN