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MGIEasy Fast Hybridization and Wash Kit

MGIEasy Fast Hybridization and Wash Kit is designed for targeted capture with rapid hybridization elution steps of DNA probes and includes hybridization elution reagents and streptavidin magnetic beads. It has broad compatibility, standardizing the hybridization process for DNA probes, and reduces hybridization time to within 2 hours while ensuring data quality, significantly shortening the liquid-phase probe hybridization capture time. By flexibly pairing with the MGIEasy series of library preparation kits, it can be used for the construction and targeted enrichment of exome libraries and panel capture libraries on the MGI high-throughput sequencing platform.


MGIEasy Fast Hybridization and Wash Kit (96 RXN)

Item No.:940-001973-00
Component kits
Product name of component kit
Part No. of component kit
Quantity of component kit in a Set
Hybridization elution reagents
MGIEasy Fast Hybridization and Wash Kit (BOX 1)
940-001826-00
1
Streptavidin magnetic beads
MGIEasy Fast Hybridization and Wash Kit (BOX 2)
940-001828-00
1

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MGIEasy Fast Hybridization and Wash Kit (16 RXN)

Item No.:940-001974-00
Component kits
Product name of component kit
Part No. of component kit
Quantity of component kit in a set
Hybridization elution reagents
MGIEasy Fast Hybridization and Wash Kit (BOX 1)
940-001825-00
1
Streptavidin magnetic beads
MGIEasy Fast Hybridization and Wash Kit (BOX 2)
940-001823-00
1

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Features

Fast hybridization
Fast hybridization

Reduce hybridization time to within 2 hours while ensuring data quality, significantly shortening the liquid-phase probe hybridization capture time. Combined with MGIEasy Fast FS Library Prep Set V2.0, it can achieve to complete the prep from gDNA to enriched libraries within one day.

​Flexible hybridization
​Flexible hybridization

Support flexible selection between 0.5 h-2 h fast hybridization and traditional overnight hybridization.

Broad compatibility
Broad compatibility
Compatible with multiple DNA probes (such as IDT, Twist, Nanod, BOKE).
Enhanced performance
Enhanced performance
Better post-capture yield, low duplication rate, high capture rate, deep and uniform coverage.
​Easy to use
​Easy to use

Simplify the operation to minimize the possibility of human errors. Automation compatible.

Fast hybridization
Fast hybridization

Reduce hybridization time to within 2 hours while ensuring data quality, significantly shortening the liquid-phase probe hybridization capture time. Combined with MGIEasy Fast FS Library Prep Set V2.0, it can achieve to complete the prep from gDNA to enriched libraries within one day.

​Flexible hybridization
​Flexible hybridization

Support flexible selection between 0.5 h-2 h fast hybridization and traditional overnight hybridization.

Broad compatibility
Broad compatibility
Compatible with multiple DNA probes (such as IDT, Twist, Nanod, BOKE).
Enhanced performance
Enhanced performance
Better post-capture yield, low duplication rate, high capture rate, deep and uniform coverage.
​Easy to use
​Easy to use

Simplify the operation to minimize the possibility of human errors. Automation compatible.

Recommended Applications

Probes and Hybridization reagent for capture Per-capture library preparation Sequencing Application

Probes: commercial DNA probes

Hybridization: MGIEasy Fast Hybridization and Wash Kit

Blocker: MGIEasy Dual Barcode Exome Capture Accessory Kit

MGIEasy Fast FS Library Prep Set V2.0

& MGIEasy Dual Barcode Circularization Kit

DNBSEQ-G400(PE100/PE150)

or

DNBSEQ-G99 (PE150)

or

DNBSEQ-T7 (PE100/PE150)

Whole exome sequencing/ target sequencing


Whole exome sequencing/target sequencing

target sequencing

MGIEasy UDB Universal Library Prep Set

MGIEasy Duplex UMI Universal Library Prep Set

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Product Specification

Applications
Hybridization capture of target regions in human samples, such as WES, small panel sequencing
Probes
commercial DNA probes (such as IDT, Twist, Nanod, BOKE)
Hybridization time
0.5h~24h
Sample type
Huamn gDNA, FFPE DNA, cfDNA
Platform Compatibility
MGISEQ-2000, DNBSEQ-G99, DNBSEQ-T7
Pre-library

pre-PCR library prepared using MGI double barcode adapters for hybridization reaction.

(It is recommended to frag the sample to be constructed into fragments with a main banddistribution of 200~300bp)

multi-plex hybridization
Compatible with single-plex and multi-plex samples hybridization (supports multi-hybridization of up to 12 samples)
Circularization
MGI universal circularization

Recommended MGI Process

Applications
Library preparation 
Sequencing 
Data analysis
  • Human WES
  • MGIEasy Fast FS Library Prep Set V2.0
  • MGIEasy Fast Hybridization and Wash Kit
  • MGIEasy Dual Barcode Exome Capture Accessory Kit
  • Other commercialized WES DNA probes
  • MGIEasy Dual Barcode Circularization Kit
  • DNBSEQ-G400
  • DNBSEQ-G99
  • DNBSEQ-T7
  • MegaBOLT Bioinformatics analysis accelerator

    -WES Germline variant calling pipeline

    -WES Somatic variant calling pipeline

  • Capture panel sequencing
  • MGIEasy Fast FS Library Prep Set V2.0
  • MGIEasy Fast Hybridization and Wash Kit
  • MGIEasy Dual Barcode Exome Capture Accessory Kit
  • Other commercialized DNA probes
  • MGIEasy Dual Barcode Circularization Kit
  • DNBSEQ-G400
  • DNBSEQ-G99
  • DNBSEQ-T7

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  • Hotline (Global)
    +86-4000-688-114
  • Email
    MGI-service@mgi-tech.com
  • Special Recommendations
  • *For research use only.

    Not for use in diagnostic procedures (except as specifically noted).
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