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MGIEasy Fast FS Library Prep Set V2.0

The MGIEasy Fast FS Library Prep Set V2.0 is designed to convert genomic DNA (gDNA) into a special library for DNBSEQ^TM sequencers and uses high quality fast fragmentase to combine the fragmentation, end-repair and add A into one step, to simplify the preparation process and significantly shorten the duration of DNA library preparation, reducing the library prep time in 2 hours.

This library prep set provides flexible workflow to be compatible with 1 ng~1 μg DNA input, supporting variety sample types, including blood, saliva, oral swabs，FFPE and precious samples with low starting amount，suitable for microbial sequencing, metagenomics sequencing, WGS and WES.

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MGIEasy Fast FS Library Prep Set V2.0 (16 RXN)

Item No.：940-001193-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast FS Library Prep Module V2.0 (16 RXN) Item No.: 940-001197-00	1
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit (16 barcode,16 RXN) Item No.: 1000022800	1
Clean Beads	MGIEasy DNA Clean Beads (3.2 mL beads) Item No.: 940-001176-00	1

MGIEasy Fast FS Library Prep Set V2.0 (96 RXN)

Item No.：940-001194-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast FS Library Prep Module V2.0 (96 RXN) Item No.: 940-001195-00	1
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit B (96 barcode,96 RXN) Item No.: 1000022802	1
Clean Beads	MGIEasy DNA Clean Beads (15 mL beads) Item No.: 940-001174-00	1

MGIEasy Fast FS Library Prep Set C V2.0 (96 RXN)

Item No.：940-001831-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast FS Library Prep Module V2.0 (96 RXN) Item No.: 940-001195-00	1
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit C (96 barcode,96 RXN) Item No.: 940-001748-00	1
Clean Beads	MGIEasy DNA Clean Beads (15 mL beads) Item No.: 940-001174-00	1

MGIEasy Fast FS Library Prep Set V2.0 (192 RXN)

Item No.：940-001196-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast FS Library Prep Module V2.0 (96 RXN) Item No.: 940-001195-00	2
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit A (96 barcode,96 RXN) Item No.: 1000022801	1
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit B (96 barcode,96 RXN) Item No.: 1000022802	1
Clean Beads	MGIEasy DNA Clean Beads (15 mL beads) Item No.: 940-001174-00	2

Features

Fast library prep workflow Combine the DNA fragmentation, end repair and add A into one step，significantly reducing the library prep time in 2 hours.	Flexible workflow to accommodate variations in sample type, DNA input amount, application Compatible with 1 ng~1 μg DNA input, supporting variety sample types, including blood, saliva, oral swabs, FFPE and precious samples with low starting amount, suitable for microbial sequencing, metagenomics sequencing, WGS and WES.
High uniform and consistent insert sizes across a wide DNA input range and sample types Use high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition, resulting in the high uniform and consistent insert sizes.	Efficient automatable workflow Compatible with automatic sample preparation systems MGISP-100 and MGISP-960 to provide automated workflows.

Fast library prep workflow

Combine the DNA fragmentation, end repair and add A into one step，significantly reducing the library prep time in 2 hours.

Flexible workflow to accommodate variations in sample type, DNA input amount, application

Compatible with 1 ng~1 μg DNA input, supporting variety sample types, including blood, saliva, oral swabs, FFPE and precious samples with low starting amount, suitable for microbial sequencing, metagenomics sequencing, WGS and WES.

High uniform and consistent insert sizes across a wide DNA input range and sample types

Use high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition, resulting in the high uniform and consistent insert sizes.

Efficient automatable workflow

Compatible with automatic sample preparation systems MGISP-100 and MGISP-960 to provide automated workflows.

Product Specification

Version	V2.0
Assay Time	~2 hrs (from gDNA to PCR library)
Input Quantity	1 ng~200 ng gDNA for small genomes (e.g. microbial, meta),25 ng~1 μg gDNA for large genomes (e.g. human, plant, animal)
Barcode	~288 Unique Dual Barcodes
Sample types	gDNA from blood, saliva, oral swabs, tissue, Microbial gDNA, Meta DNA, FFPE DNA
Species Compatibility	Human, Animal, Plant, Microbe
Applications	Human WGS，Plants & Animals WGS, Microbial WGS, Metagenomics sequencing, WES
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-G99, DNBSEQ-E25, DNBSEQ-T7, DNBSEQ-T10×4, DNBSEQ-T20×2
Recommended Read Length	SE100/PE100/PE150
Adaptation automation platform	MGISP-100(16RXN)，MGISP-960(96RXN、192RXN)

Recommended MGI Process

Applications	Library preparation	Sequencing	Data analysis
Metagenomics sequencing	MGISP-100/MGISP-960 MGIEasy Fast FS Library Prep Set V2.0 DNBSEQ OneStep DNB Make Reagent Kit V2.0(OS-DB)	DNBSEQ-G99 MGISEQ-G50 DNBSEQ-E25 MGISEQ-G400 DNBSEQ-T7 DNBSEQ-T10×4	Platform of microorganisms Fast Identification (PFI)
Human WGS	MGISP-100/MGISP-960 MGIEasy Fast FS Library Prep Set V2.0 MGIEasy Dual Barcode Circularization Kit	MGISEQ-G400 DNBSEQ-T7 DNBSEQ-T10×4 DNBSEQ-T20×2	MegaBOLT Bioinformatics Analysis Accelerator - WGS Germline variant calling pipeline - WGS Somatic variant calling pipeline
Human WES	MGISP-100/MGISP-960 MGIEasy Fast FS Library Prep Set V2.0 MGIEasy Dual Barcode Exome Capture Accessory Kit MGIEasy Exome Capture V5 Probe Set or other commercialized WES probes MGIEasy Dual Barcode Circularization Kit	MGISEQ-G400 DNBSEQ-T7	MegaBOLT Bioinformatics analysis accelerator - WES Germline variant calling pipeline - WES Somatic variant calling pipeline
Plant/Animal WGS (For Plant/Animal molecule breeding, population evolution. etc)	MGISP-100/MGISP-960 MGIEasy Fast FS Library Prep Set V2.0 MGIEasy Dual Barcode Circularization Kit	MGISEQ-G400 DNBSEQ-T7 DNBSEQ-T10×4

Related Products

MGIEasy Exome Capture V5 Probe Set

Applications: To capture the exome of human genome. It also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects, etc.

Suitable sequencing platforms: DNBSEQ-G400, DNBSEQ-T7

Reaction/kit: 16 RXN/kit

MGIEasy Dual Barcode Circularization Kit

Application: Prepare the double-stranded DNA library with MGl dual barcode adapter into a single-stranded circular DNA library for DNBSEQ seouencers. Applicable to the double-stranded DNA ibraries with MG1 dual barcode adapter from all MGl dual barcode librarv prep kits.

Platform Compatibility: All DNBSEQ

Reaction/kit: 16 RXN