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MGIEasy Fast RNA Library Prep Set

The MGIEasy Fast RNA Library Prep Set is a specifically designed RNA rapid library prep product for DNBSEQTM sequencers. The library prep set is optimized to convert 10 ng~1 μg of total RNA into a DNA library for gene expression profiling, transcriptome analysis, or detection of pathogenic microorganisms in high-throughput RNA sequencing on DNBSEQTM sequencers.

The library prep set is optimized with high quality enzyme and buffer system to combine cDNA second strand synthesis and end repair steps, which greatly shortens the time of library construction. The second strand synthesis is equipped with two kinds of buffers that can be used to prepare a common or directional library according to your needs. dTTP is replaced with dUTP in the strand-specific second strand synthesis buffer so that dUTP is incorporated into the second strand of cDNA. The high-fidelity DNA polymerase used in this kit cannot amplify the DNA template containing uracil, which achieves strand specificity.



MGIEasy Fast RNA Library Prep Set (16 RXN)

Item No.:940-000890-00
Component Kits
Component Kits Name & Item No. Quantity
Core library

MGIEasy Fast RNA Library Prep Kit (16 RXN)


Item No.: 940-000887-00

1
PCR primers & Adapter

MGIEasy UDB Primers Adapter Kit (16 barcode,16 RXN)


Item No.: 1000022800

1
Clean Beads

MGIEasy DNA Clean Beads (3.2 mL beads)


Item No.: 940-001176-00

1

>

MGIEasy Fast RNA Library Prep Set (96 RXN)

Item No.:940-000889-00
Component Kits
Product Name & Item No. Quantity
Core library

MGIEasy Fast RNA Library Prep Kit (96 RXN)


Item No.:940-000888-00

1
PCR primers & Adapter


MGIEasy UDB Primers Adapter Kit B (96 barcode,96 RXN)



Item No.:1000022802

1
Clean Beads

MGIEasy DNA Clean Beads (15 mL beads)


Item No.:940-001174-00

1

>

MGIEasy Fast RNA Library Prep Set C (96 RXN)

Item No.:940-001835-00
Component Kits
Product Name & Item No. Quantity
Core library

MGIEasy Fast RNA Library Prep Kit (96 RXN)


Item No.:940-000888-00

1
PCR primers & Adapter

MGIEasy UDB Primers Adapter Kit C (96 barcode,96 RXN)


Item No.:940-001748-00

1
Clean Beads

MGIEasy DNA Clean Beads (15 mL beads)


Item No.:940-001174-00

1

>

MGIEasy Fast RNA Library Prep Set (192 RXN)

Item No.:940-000961-00
Component Kits
Component Kits Name & Item No. Quantity
Core library

MGIEasy Fast RNA Library Prep Kit (96 RXN)


Item No.: 940-000888-00

2
PCR primers & Adapter

MGIEasy UDB Primers Adapter Kit A (96 barcode,96 RXN)


Item No.: 1000022801

1

MGIEasy UDB Primers Adapter Kit B (96 barcode,96 RXN)


Item No.: 1000022802

1
Clean Beads

MGIEasy DNA Clean Beads (15 mL beads)


Item No.: 940-001174-00

2

>

Features

Fast library prep workflow
Fast library prep workflow
Combine the cDNA second strand synthesis, end repair and dA-tailing into one step, greatly shorten the time of library prep time and minimize hands-on time.
Flexible library perp with two library types
Flexible library perp with two library types

Provide two kinds of cDNA second strand synthesis buffer, support to prepare a common or directional library.

Excellent performance
Excellent performance

High uniformity coverage across transcripts, accurate detection of strand orientation, high concordance of gene expression.

Wide range of total RNA input and multiples sample types
Wide range of total RNA input and multiples sample types

Compatible with a range of total RNA input (10 ng~1 ug) and multiples sample types, including low-quality RNA, FFPE RNA

Flexible Solutions
Flexible Solutions

Adapt to various RNA enrichment methods, sequencing read lengths,two DNB method (universal circularization or OneStep DNB) to meet

the needs of different sample types.

Fast library prep workflow
Fast library prep workflow
Combine the cDNA second strand synthesis, end repair and dA-tailing into one step, greatly shorten the time of library prep time and minimize hands-on time.
Flexible library perp with two library types
Flexible library perp with two library types

Provide two kinds of cDNA second strand synthesis buffer, support to prepare a common or directional library.

Excellent performance
Excellent performance

High uniformity coverage across transcripts, accurate detection of strand orientation, high concordance of gene expression.

Wide range of total RNA input and multiples sample types
Wide range of total RNA input and multiples sample types

Compatible with a range of total RNA input (10 ng~1 ug) and multiples sample types, including low-quality RNA, FFPE RNA

Flexible Solutions
Flexible Solutions

Adapt to various RNA enrichment methods, sequencing read lengths,two DNB method (universal circularization or OneStep DNB) to meet

the needs of different sample types.

Product Specification

Assay time
~ 4 hrs (from total RNA to PCR library)
Hands-On Time
~ 1 hrs
Input quantity
10 ng~1 μg total RNA
Barcode
~288 Unique Dual Barcodes
Sample types

Total RNA from blood, saliva, fresh tissue, FFPE RNA, Plant total RNA, Animal total RNA, Microbial total RNA, Meta RNA

Species compatibility
Human, Animal, Plant, Microbe
Applications

Whole-Transcriptome Sequencing, mRNA Sequencing, Long non-coding RNA Sequencing, RNA-Seq, Metatranscriptome Sequencing

Strand

specificity

Support strand-specificity library prep or regular library prep.

The second strand synthesis module is equipped with two buffers (dNTP buffer、dUTP buffer) to meet the need for a conventional library or strand-specific library.

Platform compatibility
DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-G99, DNBSEQ-T7, DNBSEQ-T10×4
Recommended read length

SE50/PE100/PE150

Adaptation automation platform
MGISP-100(16RXN), MGISP-960(96RXN, 192RXN), MGISP-Smart8

Recommended MGI Process

Applications
Library preparation
Sequencing
Data analysis
  • Whole-Transcriptome

    Sequencing(Human)


  • MGISP-100/MGISP-960
  • RNA enrichment: MGIEasy rRNA Depletion Kit
  • library: MGIEasy Fast RNA Library Prep Set
  • MGIEasy Dual Barcode Circularization Kit
  • DNBSEQ-G400
  • DNBSEQ-G99
  • MGISEQ-200
  • DNBSEQ-T7
  • DNBSEQ-T10×4

  • mRNA Sequencing

  • MGISP-100/MGISP-960
  • RNA enrichment: other commercial oligo dT beads reagent products
  • library: MGIEasy Fast RNA Library Prep Set
  • MGIEasy Dual Barcode Circularization Kit
  • DNBSEQ-G400
  • DNBSEQ-G99
  • MGISEQ-200
  • DNBSEQ-T7
  • DNBSEQ-T10×4

  • Metatranscriptome Sequencing

  • MGISP-100/MGISP-960
  • RNA enrichment: other commercial rRNA depletion reagent products for microorganisms

  • library: MGIEasy Fast RNA Library Prep Set

  • MGIEasy Dual Barcode Circularization Kit

  • DNBSEQ-G400
  • DNBSEQ-G99
  • MGISEQ-200
  • DNBSEQ-T7
  • DNBSEQ-T10×4
  • Platform of microorganisms Fast Identification (PFI)

>

Related Documents

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    +86-4000-688-114
  • Email
    MGI-service@mgi-tech.com
  • Special Recommendations
  • *For research use only.

    Not for use in diagnostic procedures (except as specifically noted).
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