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MGIEasy Exome Capture V4 Probe Set

MGIEasy Exome Capture V4 Probe Set is the first exome capture product that is independently designed by MGI. The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on. The probe set can be flexibly matched with MGIEasy Exome Universal Library Prep Set or MGIEasy Exome FS Library Prep Set for the construction and hybridization of libraries for MGI sequencing platforms; it can also be matched with other library preparation kits for other high throughput sequencing platforms.


MGIEasy Exome Capture V4 Probe Set(16 RXN)

Item No.:1000007745

Probes

MGIEasy Exome Capture V4 Probe(16RXN)

1000007740

Hybridization and elution

MGIEasy MGIEasy Exome Capture Elution Kit(16RXN)

1000007742
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Features

Probe regions 59Mb, covering over 20000(refGene)genes
Probe regions 59Mb, covering over 20000(refGene)genes
The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on.
Deep and comprehensive coverage
Deep and comprehensive coverage
In HGMD and ClinVar databases, MGIEasy Exome Capture V4 Probe can cover 97% and 85% of mutation sites respectively, playing an important role in disease research and diagnosis.
Stable and efficient capture rate
Stable and efficient capture rate
The capture rate is over 75%.
High consistency rate of variant detection
High consistency rate of variant detection
For cell line NA12878,the consistency rate of MGI Probe V4 is 99.2% and 87% for SNV and indels, respectively.
Compatible with various library preparation methods and sequencing platforms
Compatible with various library preparation methods and sequencing platforms
Compatible with MGIEasy Exome Universal Library Prep Kit and MGIEasy Exome FS Library Prep Kit, as well as other library preparation kits. Compatible with sequencing platforms from MGI, Illumina and Thermo Fisher.
Probe regions 59Mb, covering over 20000(refGene)genes
Probe regions 59Mb, covering over 20000(refGene)genes
The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on.
Deep and comprehensive coverage
Deep and comprehensive coverage
In HGMD and ClinVar databases, MGIEasy Exome Capture V4 Probe can cover 97% and 85% of mutation sites respectively, playing an important role in disease research and diagnosis.
Stable and efficient capture rate
Stable and efficient capture rate
The capture rate is over 75%.
High consistency rate of variant detection
High consistency rate of variant detection
For cell line NA12878,the consistency rate of MGI Probe V4 is 99.2% and 87% for SNV and indels, respectively.
Compatible with various library preparation methods and sequencing platforms
Compatible with various library preparation methods and sequencing platforms
Compatible with MGIEasy Exome Universal Library Prep Kit and MGIEasy Exome FS Library Prep Kit, as well as other library preparation kits. Compatible with sequencing platforms from MGI, Illumina and Thermo Fisher.

Recommended Applications

Library preparation set Probes for capture Compatible sequencing Read length Applications
MGIEasy Exome Capture V4 Probe Set MGIEasy Exome FS Library Prep Set (1000009658)

BGISEQ-500RS

MGISEQ-2000RS

DNBSEQ-G400RS

PE100/PE150 Whole exome sequencing
MGIEasy Exome Universal Library Prep Set PE100/PE150 Whole exome sequencing
Other library preparation kits PE100/PE150 Whole exome sequencing
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Product Specification

Product name

MGIEasy Exome Capture V4 Probe Set

Version V1.0

Packaging (Part number)

16 RXN (1000007745)

Probe lenght

90nt

Covered region size

59Mb

Databasesused for probe design

CCDS、GENCODE、RefSeq、miRBase

Covered regions

Exome regions, >99% disease-related regions

Storage

-80℃,-25℃~-18℃,Room temperature(Refer to kit label)

DNAinput 

1μg PCR product for one sample per hybridization, >250ng each sample for multiple samples per hybridizationand 1μg ≤total input≤2μg

Number of samples per hybridization

1-8

Sample types

PCR product

Compatible species

Human

Compatible sequencing platforms

Platforms from MGI,Illumina and Thermo Fisher


Read length

PE100/PE150


Detectable mutation types


SNV& Indel

Technologies

Capture+NGS

Read length

PE100/PE150

Recommended MGI Process

Applications
Library preparation
Sequencing
Bioinformation analysis
  • Whole exome sequencing

  • MGIEasy Exome Universal/ FS Library Prep Set

  • MGIEasy Exome Capture V4 Probe Set

  • BGISEQ-500RS、

    MGISEQ-2000RS、

    DNBSEQ-G400RS(PE100、PE150)

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