MGIEasy FS PCR-Free DNA Library Prep Set （96 RXN）
|Library and Circularization||
MGIEasy FS PCR-Free DNA Library Prep Kit
|Adapter||MGIEasy PF Adapters-96（plate）Kit||
MGIEasy DNA Clean Beads
MGIEasy FS PCR-Free DNA Library Prep Set （16 RXN）
|Library and Circularization||MGIEasy FS PCR-Free DNA Library Prep Kit||1000013458|
MGIEasy PF Adapters-16（tube）Kit
|Beads||MGIEasy DNA Clean Beads||1000005278|
Good compatibility for different species fragmentation
Use a high-quality，low bias fragmentase for different species and input amounts shearing with the same incubation condition can get the consistent and concentrated fragment range.
Stable library yield
The modified reaction system increases the library quality and results in a stable library yield.
Simple and automated workflow
Library construction can be completed within 4.5 hours. Compatible with automatic sample preparation systems to provide an automated solution.
No amplification error accumulation
WGS PCR-free library preparation in combination with sequencing by the MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity.
Good coverage uniformity
The genome coverage of FS PCR-free achieved comparable performance to Covaris PCR-free. The FS PCR-free technique covers the GC-rich, promoter and repetitive regions. Significantly better than traditional WGS methods.
Excellent variant performance
The variant detection performance of FS PCR-free is similar to Covaris PCR-free, and better than traditional PCR, especially InDels.
|Product Name||MGIEasy FS PCR-Free DNA Library Prep Set|
|Application||Whole genome sequencing|
|The version of Set||
|Configuration (Cat.)||16 RXN(1000013454), 96 RXN(1000013455)|
|Validity period||9 months|
|Assay Time||~4.5 hours|
|Input Quantity||200 ng - 1 μg gDNA|
|Species Compatibility||Human, animals, plants, fungi, bacteria, metagenomics, etc.|
|Recommended Insert Size||350-475 bp|
|Platform Compatibility||MGISEQ-2000、MGISEQ-200、DNBSEQ-G400、DNBSEQ-G50、 BGISEQ-500|
|Recommended Read Length||PE100, PE150|
Recommended MGI Process
MGIEasy PCR-Free Library Prep Set is specifically designed for making WGS libraries for MGI High-throughput Sequencing Platforms. This library prep set is optimized to convert 120-200 ng size selected fragmented DNA into a customized library. MGI WGS PCR-free library preparation, combined with MGI DNBSEQTM genetic sequencer, is the true PCR-free NGS workflow without PCR amplification, resulting in no error accumulation and higher data accuracy.
1000013454，1000013455 MGIEasy FS PCR-Free DNA Library Prep Set User Manual-A1 This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used.
- Demo DataRead Length:PE150 Sample:NA12878 Platform:DNBSEQ-G400/MGISEQ-2000
Demo Data 1 Demo Data 2 Demo Data 3 Demo Data 4
- SAFETY DATA SHEETS（SDS）