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MGIEasy Exome Capture V5 Probe Set

MGIEasy Exome Capture V5 Probe Set not only covers the regions of traditional exome probes, but also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects etc. The primary targets with a design size of 69Mb include:CCDS, GENCODE, RefSeq, miRbase and MGI Clinical Database. As a universal exome capture probe set, it is compatible with both of MGI and other NGS platforms.


MGIEasy Exome Capture V5 Probe Set(16 RXN)

Item No.:1000007746

Probes

MGIEasy Exome Capture V5 Probe(16RXN)

1000007741

Hybridization and elution

MGIEasy MGIEasy Exome Capture Elution Kit(16RXN)

1000007742
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Features

Probe regions 69Mb
Probe regions 69Mb
The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on. It also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects etc.
More comprehensive gene coverage
More comprehensive gene coverage
455 genes can be 100% covered.
Superior coverage and uniformity
Superior coverage and uniformity
When the mean sequencing depth is 100X, 96% of the regions can reach coverage of 20X.
Stable and efficient capture rate
Stable and efficient capture rate
The capture rate is over >60% (by reads).
Probe regions 69Mb
Probe regions 69Mb
The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on. It also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects etc.
More comprehensive gene coverage
More comprehensive gene coverage
455 genes can be 100% covered.
Superior coverage and uniformity
Superior coverage and uniformity
When the mean sequencing depth is 100X, 96% of the regions can reach coverage of 20X.
Stable and efficient capture rate
Stable and efficient capture rate
The capture rate is over >60% (by reads).

Recommended Applications

Library preparation set Probes for capture Compatible sequencing Read length Applications
MGIEasy Exome Capture V5 Probe Set MGIEasy Exome FS Library Prep Set (1000009658)

BGISEQ-500RS

MGISEQ-2000RS

DNBSEQ-G400RS

PE100/PE150 Whole exome sequencing
MGIEasy Exome Universal Library Prep Set PE100/PE150 Whole exome sequencing
Other library preparation kits PE100/PE150 Whole exome sequencing
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Product Specification

Kit name

MGIEasy Exome Capture V5 Probe Set

Version V1.0

Packaging (Part number)

16 RXN (1000007746)

Probe lenght

90nt

Covered region size

69Mb

Databases used for probe design

CCDS、GENCODE、RefSeq、miRBase、MGI Clinical Database

Covered regions

Traditional exome regions and various diseases related regions

Storage

-80℃,-25℃~-18℃,Room temperature(Refer to kit label)

DNA input

1μg PCR product for one sample per hybridization, >250ng each sample for multiple samples per hybridization and 1μg ≤total input≤2μg

Number of samples per hybridization

1-8

Sample types

PCR product

Compatible species

Human

Compatible sequencing platforms

Platforms from MGI,Illumina and Thermo Fisher

Read length

PE100/PE150

Detectable mutation types

SNV& Indel
Technologies
Capture+NGS

Recommended MGI Process

Applications
Library preparation
Sequencing
Bioinformation analysis
  • Whole exome sequencing

  • MGIEasy Exome Universal/ FS Library Prep Set

  • MGIEasy Exome Capture V5 Probe Set

  • BGISEQ-500RS、

    MGISEQ-2000RS、

    DNBSEQ-G400RS(PE100、PE150)

>

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