MGIEasy Exome Capture V5 Probe Set(16 RXN)
Probes |
MGIEasy Exome Capture V5 Probe(16RXN) |
1000007741 |
Hybridization and elution |
MGIEasy Exome Capture Hybridization and Wash Kit (Box 1) (16RXN) |
940-000168-00 |
Hybridization and elution |
MGIEasy Exome Capture Hybridization and Wash Kit (Box 2) (16RXN) |
940-000169-00 |
Features
Probe regions 69Mb The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on. It also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects etc. | More comprehensive gene coverage 455 genes can be 100% covered. |
Superior coverage and uniformity When the mean sequencing depth is 100X, 96% of the regions can reach coverage of 20X. | Stable and efficient capture rate The capture rate is over >60% (by reads). |
Recommended Applications
Probes for capture | Library preparation set | Compatible sequencing | Read length | Applications |
---|---|---|---|---|
MGIEasy Exome Capture V5 Probe Set | MGIEasy Exome FS Library Prep Set (1000009658) |
DNBSEQ-T7 |
PE100/PE150 |
Whole exome sequencing |
MGIEasy Exome Universal Library Prep Set | PE100/PE150 |
Whole exome sequencing |
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Other library preparation kits | PE100/PE150 |
Whole exome sequencing |
Product Specification
Kit name |
MGIEasy Exome Capture V5 Probe Set |
Probe lenght |
90nt |
Covered region size |
69Mb |
Databases used for probe design |
CCDS, GENCODE, RefSeq, miRBase, MGI Clinical Database |
Covered regions |
Traditional exome regions and various diseases related regions |
Storage |
-80℃,-25℃~-18℃,Room temperature(Refer to kit label) |
DNA input |
1μg PCR product for one sample per hybridization, >250ng each sample for multiple samples per hybridization and 1μg ≤total input≤2μg |
Number of samples per hybridization |
1-8 |
Sample types |
PCR product |
Compatible species |
Human |
Compatible sequencing platforms |
Platforms from MGI,Illumina and Thermo Fisher |
Read length |
PE100/PE150 |
Detectable mutation types |
SNV & Indel |
Technologies |
Capture+NGS |
Recommended MGI Process
Applications
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Library preparation
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Sequencing
|
Bioinformation analysis
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Related Products
Related Documents
- Materials
MGIEasy Exome Capture V5 Probe Set Datasheet Download
MGIEasy Exome Capture V5 Probe Set User Manual 1.0
This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used. - SDS