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MGIEasy Fast FS Library Prep Set V2.0

The MGIEasy Fast FS Library Prep Set V2.0 is designed to convert genomic DNA (gDNA) into a special library for DNBSEQTM sequencers and combines the fragmentation, end-repair and add A into one step, to simplify the preparation process and significantly shorten the duration of DNA library preparation, reducing the library prep time in 2 hours. 

This library prep set provides flexible workflow to be compatible with 1 ng~1 μg DNA input, supporting variety sample types, including blood, saliva, oral swabs, FFPE and precious samples with low starting amount, suitable for microbial sequencing, metagenomics sequencing, WGS and WES.



MGIEasy Fast FS Library Prep Set V2.0 (16 RXN)

Item No.:940-001193-00
Component Kits
Component Kits Name & Item No. Quantity
Core library

MGIEasy Fast FS Library Prep Module V2.0 (16 RXN)


Item No.: 940-001197-00

1
PCR primers & Adapter

MGIEasy UDB Primers Adapter Kit (16 barcode,16 RXN)


Item No.: 1000022800

1
Clean Beads

MGIEasy DNA Clean Beads (3.2 mL beads)


Item No.: 940-001176-00

1

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MGIEasy Fast FS Library Prep Set V2.0 (96 RXN)

Item No.:940-001194-00
Component Kits
Component Kits Name & Item No. Quantity
Core library

MGIEasy Fast FS Library Prep Module V2.0 (96 RXN)


Item No.: 940-001195-00

1
PCR primers & Adapter

MGIEasy UDB Primers Adapter Kit B (96 barcode,96 RXN)


Item No.: 1000022802

1
Clean Beads

MGIEasy DNA Clean Beads (15 mL beads)


Item No.: 940-001174-00

1

>

MGIEasy Fast FS Library Prep Set C V2.0 (96 RXN)

Item No.:940-001831-00
Component Kits
Component Kits Name & Item No. Quantity
Core library

MGIEasy Fast FS Library Prep Module V2.0 (96 RXN)


Item No.: 940-001195-00

1
PCR primers & Adapter

MGIEasy UDB Primers Adapter Kit C (96 barcode,96 RXN)


Item No.: 940-001748-00

1
Clean Beads

MGIEasy DNA Clean Beads (15 mL beads)


Item No.: 940-001174-00

1

>

MGIEasy Fast FS Library Prep Set V2.0 (192 RXN)

Item No.:940-001196-00
Component Kits
Component Kits Name & Item No. Quantity
Core library

MGIEasy Fast FS Library Prep Module V2.0 (96 RXN)


Item No.: 940-001195-00

2
PCR primers & Adapter

MGIEasy UDB Primers Adapter Kit A (96 barcode,96 RXN)


Item No.: 1000022801

1

MGIEasy UDB Primers Adapter Kit B (96 barcode,96 RXN)


Item No.: 1000022802
1
Clean Beads

MGIEasy DNA Clean Beads (15 mL beads)


Item No.: 940-001174-00

2

>

Features

Fast library prep workflow
Fast library prep workflow
Combine the DNA fragmentation, end repair and add A into one step,significantly reducing the library prep time in 2 hours. 
Flexible workflow to accommodate variations in sample type, DNA input amount, application
Flexible workflow to accommodate variations in sample type, DNA input amount, application
Compatible with 1 ng~1 μg DNA input, supporting variety sample types, including blood, saliva, oral swabs, FFPE and precious samples with low starting amount, suitable for microbial sequencing, metagenomics sequencing, WGS and WES.
High uniform and consistent insert sizes across a wide DNA input range and sample types
High uniform and consistent insert sizes across a wide DNA input range and sample types

Use high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition, resulting in the high uniform and consistent insert sizes.

Efficient automatable workflow
Efficient automatable workflow
Compatible with automatic sample preparation systems MGISP-100 and MGISP-960 to provide automated workflows.
Fast library prep workflow
Fast library prep workflow
Combine the DNA fragmentation, end repair and add A into one step,significantly reducing the library prep time in 2 hours. 
Flexible workflow to accommodate variations in sample type, DNA input amount, application
Flexible workflow to accommodate variations in sample type, DNA input amount, application
Compatible with 1 ng~1 μg DNA input, supporting variety sample types, including blood, saliva, oral swabs, FFPE and precious samples with low starting amount, suitable for microbial sequencing, metagenomics sequencing, WGS and WES.
High uniform and consistent insert sizes across a wide DNA input range and sample types
High uniform and consistent insert sizes across a wide DNA input range and sample types

Use high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition, resulting in the high uniform and consistent insert sizes.

Efficient automatable workflow
Efficient automatable workflow
Compatible with automatic sample preparation systems MGISP-100 and MGISP-960 to provide automated workflows.

Product Specification

Version V2.0
Assay Time
~2 hrs (from gDNA to PCR library)
Input Quantity

1 ng~200 ng gDNA for small genomes (e.g. microbial, meta),25 ng~1 μg gDNA for large genomes (e.g. human, plant, animal)

Barcode
~288 Unique Dual Barcodes
Sample types

gDNA from blood, saliva, oral swabs, tissue, Microbial gDNA, Meta DNA, FFPE DNA

Species Compatibility
Human, Animal, Plant, Microbe
Applications

Human WGS,Plants & Animals WGS, Microbial WGS, Metagenomics sequencing, WES

Platform Compatibility

DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-G99, DNBSEQ-E25, DNBSEQ-T7, DNBSEQ-T10×4, DNBSEQ-T20×2

Recommended Read Length
SE100/PE100/PE150
Adaptation automation platform
MGISP-100(16RXN),MGISP-960(96RXN、192RXN)

Recommended MGI Process

Applications
Library preparation
Sequencing
Data analysis
  • Metagenomics sequencing

  • MGISP-100/MGISP-960
  • MGIEasy Fast FS Library Prep Set V2.0

  • DNBSEQ OneStep DNB Make Reagent Kit V2.0(OS-DB) (940-000036-00)

  • DNBSEQ-G99
  • MGISEQ-G50
  • DNBSEQ-E25

  • MGISEQ-G400

  • DNBSEQ-T7
  • DNBSEQ-T10×4
  • Platform of microorganisms Fast Identification (PFI)
  • Human WGS

  • MGISP-100/MGISP-960
  • MGIEasy Fast FS Library Prep Set V2.0

  • MGIEasy Dual Barcode Circularization Kit

  • MGISEQ-G400
  • DNBSEQ-T7
  • DNBSEQ-T10×4
  • DNBSEQ-T20×2
  • MegaBOLT Bioinformatics Analysis Accelerator

    - WGS Germline variant calling pipeline

    - WGS Somatic variant calling pipeline

  • Human WES

  • MGISP-100/MGISP-960
  • MGIEasy Fast FS Library Prep Set V2.0

  • MGIEasy Dual Barcode Exome Capture Accessory Kit

  • MGIEasy Exome Capture V5 Probe Set or other commercialized WES probes

  • MGIEasy Dual Barcode Circularization Kit

  • MGISEQ-G400
  • DNBSEQ-T7
  • MegaBOLT Bioinformatics analysis accelerator

    - WES Germline variant calling pipeline

    - WES Somatic variant calling pipeline

  • Plant/Animal WGS (For Plant/Animal molecule breeding, population evolution. etc)

  • MGISP-100/MGISP-960
  • MGIEasy Fast FS Library Prep Set V2.0

  • MGIEasy Dual Barcode Circularization Kit

  • MGISEQ-G400
  • DNBSEQ-T7
  • DNBSEQ-T10×4

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  • *For research use only.

    Not for use in diagnostic procedures (except as specifically noted).
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