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HIV-1 Drug Resistance Sequencing Products Package

Drug resistance mutations of the HIV virus leading to antiretroviral resistance are the most common causes of treatment failure in HIV patients. Timely drug resistance testing and monitoring, as well as understanding the resistance status of individuals and populations, play a crucial role in the prevention and treatment of AIDS.

MGI's HIV-1 Drug Resistance Sequencing Products Package is based on self-developed library preparation reagents, automated sample preparation systems, sequencer, and analysis software. It covers the entire process from viral RNA to reporting, enabling rapid and accurate detection of HIV-1 virus in samples.

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HIV-1 Drug Resistance Sequencing Products Package

Item No.：

900-000609-00	DNBSEQ-G99ARS sequencer
900-000537-00	DNBSEQ-E25RS sequencer
900-000354-00	DNBSEQ-G50 sequencer
900-000206-00	MGISP-100RS Automated Sample Prep System
900-000147-00	MGISP-960RS Automated Sample Prep System
900-000152-00	MGISP-960RS Automated Sample Prep System
940-000722-00	ATOPlex HIV-1 Amplification Kit (16 RXN)
940-000019-00	MGIEasy Fast PCR-FREE FS DNA Library Prep Set (16 RXN)
940-000021-00	MGIEasy Fast PCR-FREE FS DNA Library Prep Set (96 RXN)
1000005278	MGIEasy DNA Clean Beads (8 mL/tube)
1000026466	DNBSEQ OneStep DNB Make Reagent Kit (4 RXN)
1000020834	CPAS barcode primer 3
940-000410-00	High-throughput Sequencing Set (G99 SM FCL PE150)
940-001269-00	High-throughput Sequencing Set (G99 FCL PE150)
1000019862	DNBSEQ-G50RS High-throughput Rapid Sequencing Set (FCS PE150)
1000019858	DNBSEQ-G50RS High-throughput Sequencing Set (FCL PE150)
940-000567-00	DNBSEQ-E25RS High-throughput Sequencing Set (FCL PE150)
970-000281-00	HIV-GenomePro Software
900-000598-00	HIV-GenomePro Platform

Recommended Applications

Features

Covering the main drug-resistant regions Only one panel covered three main drug resistance regions of HIV-1, protease (PR), reverse transcriptase (RT), and integrase (IN);	Short TAT Combined with the DNBSEQ-G99 sequencer, from RNA to report, can be completed within 20.5 hours for 16 samples;
Work for low frequency drug resistance Based on deep sequencing, capable of detecting drug resistance frequency less than 20%, which cannot be detected by the Sanger;	Automation friendly Combined with the MGISP-100, MGISP-960 and HIV-GenomePro platform, can achieve automated library prep and data analysis, save labor;
Various analysis functions Features genotyping, genome assembly, mutation detection, and evolutionary tracing. Based on the Stanford HIVdb, it can precisely analyze and annotate the drug resistance. Additionally, by merging multiple samples sequences, HIV-trace analysis can be conducted to obtain a visual molecular network.

Product Specification

Intended use	HIV-1 genotyping and drug resistance detection
Target region	PR, RT, IN genes
Sample type	Viral RNA from plasma, serum and cultured strains
Sequencer	DNBSEQ-G99/E25/G50
Recommended read length	PE150
Recommended data volume	1 M PE150 Reads
Recommended sample input	≥100 copies per rxn
Recommended throughput	16 samples/FC for DNBSEQ-E25 60 samples/FC for DNBSEQ-G99 60 samples/FC for DNBSEQ-G50 FCS 300 samples/FC for DNBSEQ-G50 FCL
TAT for library prep	＜8.5 h for 16 samples
TAT for analysis	＜2 h for 16 samples by DNBSEQ G99ARS ≈1h for 16 samples by HIV GenomePro platform