|Product Name||MGIEasy stLFR Library Prep Kit|
|Shelf life||Six months|
|Input material||Genome DNA|
|Type of samples||High molecular weight DNA|
|Requirements for DNA sample||
The DNA required for stLFR library needs to be as large as it can be. The larger the DNA is the better the performance will be. Usually the DNA isolated with methods suggested below will be with a main band greater than 100 kb when analyzing with pulse-field electrophoresis. The suggested DNA isolated kits are Qiagen MagAttract HMW DNA Kit (Cat No./ID: 67563) or Agilent Technologies RECOVEREASE DNA ISOLATION KIT (Catalog No.50-126-011) The required concentration of DNA should be more than1 ng/ul and the total amount should be more than 500ng.
|Library process||Shear DNA with enzyme, perform DNA co-barcoding, introduce another adapter, amplify with PCR and make DNB|
|Sequencing read length suggested||PE100+42|
|Sequencing platforms suggested||BGISEQ-500RS、MGISEQ-2000RS|
|Suggested coverage/Raw reads number||For human resequencing purpose: 30X， ~650M raw reads (PE100)|
|Data Sheet||MGIEasy stLFR solution Download|
1000005622-MGIEasy stLFR Library Prep Kit User Manual Download
This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used.
Read Length：PE100 Sample：NA12878 Platform：MGISEQ-2000
I Sample Information Fastq Download
II Sample Information Fastq Download