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ATOPlex RNA Library Prep Set

ATOPlex RNA Library Prep Set includes RT and two-steps PCR that convert viral RNA to sequence libraries in 5 hours. With unique technology, ATOPlex performs high specificity of amplification and low dimer rate. Moreover, the ATOPlex kit includes clean system and spike-in system which minimizes cross-contamination risk and improves the accuracy of virus loads quantification. It is suitable for population-scale virus detection, surveillance, and tracing.


ATOPlex RNA Library Prep Set

Item No.:1000023556

RT and PCR Reagment


ATOPlex RNA Universal Library Preparation Module


1000021624

Primer of SARS-CoV-2

Internal Control Primer Pool
1000021625

Dual Barcode Primer

ATOPlex Dual Barcode Primer Module (01-96)

1000021626

Dual Barcode Balance Library

ATOPlex Dual Barcoded Balance Library Reagent

1000022270

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Features

Ultra-sensitive Detection
Ultra-sensitive Detection

Ultra-sensitive Detection with as Low as 10 Copies/mL Viral Loads


Full-length Genome Coverage
Full-length Genome Coverage

>99% Full-length Genome Coverage with Challenge Sample


Simple operation
Simple operation
Easy Protocol with RT and Two-step PCR in One Tube
Accurate quantification
Accurate quantification
Accurate Quantification by Spike-in Control
Ultra-sensitive Detection
Ultra-sensitive Detection

Ultra-sensitive Detection with as Low as 10 Copies/mL Viral Loads


Full-length Genome Coverage
Full-length Genome Coverage

>99% Full-length Genome Coverage with Challenge Sample


Simple operation
Simple operation
Easy Protocol with RT and Two-step PCR in One Tube
Accurate quantification
Accurate quantification
Accurate Quantification by Spike-in Control

Product Specification

Product Name

ATOPlex RNA Library Prep Set

Configuration 96 Preps/kit
Sample Types

Total Nucleic Acid from Nasopharyngeal Swabs, BALF, etc

Application 

Surveillance, Variation and Evolution Analysis of SARS-CoV-2

Detection Region
SARS-CoV-2 Full Length
Amplicons Size 106-199 bps
Amplicons Numbles
273 Amplicons in one tube
cDNA input

>10 copies genome for full length,>10 copies/mL for detection

Variant Types

SNP,InDel

Total Time(sample to library)
5 hrs
Uniformity(0.1X)
95%
On Target Aligned Reads
≥95%
Coverage
≥99%
Recommend Sequence Type
PE100 for Full Length Genome,SE50 for Detection
Recommend Total Reads
0.5-3 M Reads/sample

Recommended MGI Process

Applications
Library preparation
Sequencing
Bioinformation analysis
  • Surveillance, Variation and Evolution Analysis of SARS-CoV-2

  • ATOPlex RNA Library Prep Set


  • Fast identification of pathogenic infections(PFI) and MGAP(Microbial Genome Analysis Pipeline)
  • MGI-SARS-CoV-2 pipeline

  • ATOPlex SARS-CoV-2 software

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