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MGIEasy RNA Exome Application

MGIEasy RNA Exome is an efficient package for targeted quantification of the coding regions of the human transcriptome. By combining directional library prep and exome capture technologies, it involves conversion of total RNA into cDNA while keeping the strand direction information, targeted capture of exon sequences, and generation of libraries for sequencing on MGI high-throughput genome sequencing platforms. MGIEasy RNA Exome provides an efficient and complete package for mRNA expression quantification of FFPE and other types of degraded samples.

MGIEasy RNA Exome is designed specifically for degraded RNA samples, by capturing only the coding regions from total RNA, it can help improve the accuracy of mRNA quantification. It requires much less data output to achieve the same required sequencing depth for the target coding regions.

MGIEasy RNA Exome Pack

age includes MGIEasy RNA Directional Library Prep Set, MGIEasy Exome Capture V5 Probe Set, and MGIEasy Exome Capture Accessory Kit. This guide will introduce the details of MGIEasy RNA Exome library preparation technology.

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MGIEasy RNA Directional Library Prep Set（16 RXN）

Item No.：1000006385

Library	MGIEasy RNA Directional Library Prep Kit V2.0（16 RXN）	1000005270
Adapters	MGIEasy DNA Adapters-16(Tubes) Kit V1.0（16 RXN, 16 barcodes）	1000005284
Clean Beads	MGIEasy DNA Clean Beads	1000005278
Circularization	MGIEasy Circularization Module V2.0	1000005260

MGIEasy Exome Capture V5 Probe Set（16 RXN）

Item No.：940-000187-00

Probes	MGIEasy Exome Capture V5 Probe（16RXN）	1000007741
Hybridization and elution	MGIEasy Exome Capture Hybridization and Wash Kit (Box 1) （16RXN）	940-000168-00
Hybridization and elution	MGIEasy Exome Capture Hybridization and Wash Kit (Box 2) （16RXN）	940-000169-00

MGIEasy Exome Capture Accessory Kit（16 RXN）

Item No.：1000007743

Features

Low input amount As little as 10 ng total input RNA. Libraries show stable performance across various input amounts.	Suitable for degraded samples High quality data can still be obtained for FFPE and other types of degraded samples.
Focus on the RNA coding region Targeted capture of human exome regions from total RNA means less data output required to achieve the same sequencing depth at the target regions.	High repeatability The repeatability for low quality samples is above 0.99.

Low input amount

As little as 10 ng total input RNA. Libraries show stable performance across various input amounts.

Suitable for degraded samples

High quality data can still be obtained for FFPE and other types of degraded samples.

Focus on the RNA coding region

Targeted capture of human exome regions from total RNA means less data output required to achieve the same sequencing depth at the target regions.

High repeatability

The repeatability for low quality samples is above 0.99.

Product Specification

Application name	MGIEasy RNA Exome
Instruction	MGIEasy RNA Exome Instructions
Item information	MGIEasy RNA Directional Library Prep Set MGIEasy Exome Capture V5 Probe Set MGIEasy Exome Capture Accessory Kit
Reaction/kit	16 RXN
Input Quantity	10 ng-100 ng total RNA
Sample types	Fresh Frozen tissues, FFPE samples
Species Compatibility	Human
Assay Time	32 hrs
Applications	RNA-Seq
Platform Compatibility	DNBSEQ-G400
Recommended Read Length	PE100
Recommended sequencing data per sample	PE100 / 25M reads

Recommended MGI Process

Applications	Library preparation	Sequencing	Bioinformation analysis
RNA Exome lncRNA Sequencing	MGISP-100 MGISP-960 MGIEasy RNA Directional Library Prep Set + MGIEasy Exome Capture V5 Probe Set + MGIEasy Exome Capture Accessory Kit	DNBSEQ-G400*（PE100）