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MGIEasy RNA Exome Application

MGIEasy RNA Exome is an efficient solution for targeted quantification of the coding regions of the human transcriptome. By combining directional library prep and exome capture technologies, it involves conversion of total RNA into cDNA while keeping the strand direction information, targeted capture of exon sequences, and generation of libraries for sequencing on MGI high-throughput genome sequencing platforms. MGIEasy RNA Exome provides an efficient and complete solution for mRNA expression quantification of FFPE and other types of degraded samples.

MGIEasy RNA Exome is designed specifically for degraded RNA samples, by capturing only the coding regions from total RNA, it can help improve the accuracy of mRNA quantification. It requires much less data output to achieve the same required sequencing depth for the target coding regions. 

MGIEasy RNA Exome Solution includes MGIEasy RNA Directional Library Prep Set, MGIEasy Exome Capture V5 Probe Set, and MGIEasy Exome Capture Accessory Kit. This guide will introduce the details of MGIEasy RNA Exome library preparation technology.



MGIEasy RNA Directional Library Prep Set(16 RXN)

Item No.:1000006385
Library MGIEasy RNA Directional Library Prep Kit V2.0(16 RXN) 1000005270
Adapters MGIEasy DNA Adapters-16(Tubes) Kit V1.0(16 RXN, 16 barcodes) 1000005284
Clean Beads MGIEasy DNA Clean Beads 1000005278
Circularization MGIEasy Circularization Module V2.0 1000005260
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MGIEasy Exome Capture V5 Probe Set(16 RXN)

Item No.:1000007746
Probe MGIEasy Exome Capture V5 Probe Kit V1.0(16 RXN) 1000007741

Hybridization and Wash Buffer

MGIEasy Exome Capture Hybridization and Wash Kit V1.0(16 RXN)

1000007742
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MGIEasy Exome Capture Accessory Kit(16 RXN)

Item No.:1000007743
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Features

Low input amount
Low input amount
As little as 10 ng total input RNA. Libraries show stable performance across various input amounts.
Suitable for degraded samples
Suitable for degraded samples
High quality data can still be obtained for FFPE and other types of degraded samples.
Focus on the RNA coding region
Focus on the RNA coding region
Targeted capture of human exome regions from total RNA means less data output required to achieve the same sequencing depth at the target regions.
High repeatability
High repeatability
The repeatability for low quality samples is above 0.99.
Low input amount
Low input amount
As little as 10 ng total input RNA. Libraries show stable performance across various input amounts.
Suitable for degraded samples
Suitable for degraded samples
High quality data can still be obtained for FFPE and other types of degraded samples.
Focus on the RNA coding region
Focus on the RNA coding region
Targeted capture of human exome regions from total RNA means less data output required to achieve the same sequencing depth at the target regions.
High repeatability
High repeatability
The repeatability for low quality samples is above 0.99.

Product Specification

Application name

MGIEasy RNA Exome

Instruction MGIEasy RNA Exome Instructions

Item information

MGIEasy RNA Directional Library Prep Set (1000006385)

MGIEasy Exome Capture V5 Probe Set(1000007746)

MGIEasy Exome Capture Accessory Kit(1000007743)

Reaction/kit 16 RXN

Input Quantity

10 ng-100 ng total RNA

Sample types

Fresh Frozen tissues, FFPE samples

Species Compatibility

Human

Assay Time

32 h
Applications

RNA-Seq

Platform Compatibility

BGISEQ-500, MGISEQ-2000, DNBSEQ-G400

Recommended Read Length

PE100

Recommended sequencing data per sample

PE100 / 25M reads

Recommended MGI Process

Applications
Library preparation
Sequencing 
Bioinformation analysis
  • RNA Exome lncRNA Sequencing

  • MGISP-100
  • MGISP-960
  • MGIEasy RNA Directional Library Prep Set  

    + MGIEasy Exome Capture V5 Probe Set

    + MGIEasy Exome Capture Accessory Kit

  • MGISEQ-2000 / DNBSEQ-G400(PE100)


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