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MGIEasy RNA Exome Application

MGIEasy RNA Exome is an efficient package for targeted quantification of the coding regions of the human transcriptome. By combining directional library prep and exome capture technologies, it involves conversion of total RNA into cDNA while keeping the strand direction information, targeted capture of exon sequences, and generation of libraries for sequencing on MGI high-throughput genome sequencing platforms. 

MGIEasy RNA Exome Pack age includes MGIEasy RNA Directional Library Prep Set, MGIEasy Exome Capture V5 Probe Set, and MGIEasy Exome Capture Accessory Kit. This guide will introduce the details of MGIEasy RNA Exome library preparation technology.


*The MGIEasy RNA Directional Library Prep Set have been discontinued. To find an alternative reagent, we recommend using our MGIEasy Fast RNA Library Prep Set. For more details, please consult your local sales representative.



MGIEasy RNA Directional Library Prep Set(16 RXN)

Item No.:1000006385
Library MGIEasy RNA Directional Library Prep Kit V2.0(16 RXN) 1000005270
Adapters MGIEasy DNA Adapters-16(Tubes) Kit V1.0(16 RXN, 16 barcodes) 1000005284
Clean Beads MGIEasy DNA Clean Beads 1000005278
Circularization MGIEasy Circularization Module V2.0 1000005260
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MGIEasy Exome Capture V5 Probe Set(16 RXN)

Item No.:940-000187-00

Probes

MGIEasy Exome Capture V5 Probe(16RXN)

1000007741

Hybridization and elution

MGIEasy Exome Capture Hybridization and Wash Kit (Box 1) (16RXN)

940-000168-00

Hybridization and elution

MGIEasy Exome Capture Hybridization and Wash Kit (Box 2) (16RXN)

940-000169-00
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MGIEasy Exome Capture Accessory Kit(16 RXN)

Item No.:1000007743
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Product Specification

Application name

MGIEasy RNA Exome

Instruction MGIEasy RNA Exome Instructions

Item information

MGIEasy RNA Directional Library Prep Set 

MGIEasy Exome Capture V5 Probe Set

MGIEasy Exome Capture Accessory Kit

Reaction/kit 16 RXN

Input Quantity

10 ng-100 ng total RNA

Sample types

Fresh Frozen tissues, FFPE samples

Species Compatibility

Human

Assay Time

32 hrs
Applications

RNA-Seq

Platform Compatibility

DNBSEQ-G400

Recommended Read Length

PE100

Recommended sequencing data per sample

PE100 / 25M reads

  • Hotline (Global)
    +86-4000-688-114
  • Email
    MGI-service@mgi-tech.com
  • Special Recommendations
  • *For research use only.

    Not for use in diagnostic procedures (except as specifically noted).
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