Go Back / Location:Home / Products

MGIEasy RNA Directional Library Prep Set

MGIEasy RNA Directional Library Prep Set provides an efficient solution for generating libraries suitable for MGI high-throughput sequencing platforms from total RNA. It can be used to identify RNA stranded information that from which of the two DNA strands a given RNA transcript originates. Identifying strand origin increases the percentage of reads that align to the transcriptome and provides more accurate information for studies in regulation of gene expression and gene functional analysis. Maintaining strand orientation also allows identification of antisense expression and novel genes.




MGIEasy RNA Directional Library Prep Set(96 RXN)

Item No.:1000006386
Library

MGIEasy RNA Directional Library Prep Kit V2.0(96 RXN)

1000005270
Adapters

MGIEasy DNA Adapters-96(Plate) Kit V1.0(96 RXN, 96 barcodes)

1000005282
Clean Beads
MGIEasy DNA Clean Beads
1000005279
Circularization
MGIEasy Circularization Module V2.0
1000005260
>

MGIEasy RNA Directional Library Prep Set(16 RXN)

Item No.:1000006385
Library

MGIEasy RNA Directional Library Prep Kit V2.0(16 RXN)

1000005270
Adapters

MGIEasy DNA Adapters-16(Tubes) Kit V1.0(16 RXN, 16 barcodes)

1000005284
Clean Beads
MGIEasy DNA Clean Beads
1000005278
Circularization
MGIEasy Circularization Module V2.0
1000005260
>

Features

Wide range of total RNA input
Wide range of total RNA input
Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.
Compatible with various sample types
Compatible with various sample types
Including low-quality RNA, FFPE RNA and plasma samples.
Excellent coverage quality
Excellent coverage quality
Accurate and comprehensive mapping of transcripts.
High uniformity
High uniformity
Enables high uniform from transcripts 5 'to 3' ends and superior 3’end coverage.
Accurate detection of strand orientation
Accurate detection of strand orientation
Identify transcript origin, detect antisense transcripts, enhances transcript annotation.
Easy to Use
Easy to Use
Simple protocol and short operation time, suitable for automated library preparation instrument.
Flexible Solutions
Flexible Solutions
Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.
Wide range of total RNA input
Wide range of total RNA input
Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.
Compatible with various sample types
Compatible with various sample types
Including low-quality RNA, FFPE RNA and plasma samples.
Excellent coverage quality
Excellent coverage quality
Accurate and comprehensive mapping of transcripts.
High uniformity
High uniformity
Enables high uniform from transcripts 5 'to 3' ends and superior 3’end coverage.
Accurate detection of strand orientation
Accurate detection of strand orientation
Identify transcript origin, detect antisense transcripts, enhances transcript annotation.
Easy to Use
Easy to Use
Simple protocol and short operation time, suitable for automated library preparation instrument.
Flexible Solutions
Flexible Solutions
Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.

Recommended Applications

Sample type RNA enrichment method Read length
Applications
Eukaryotic total RNA with high integrity Poly(A)+ mRNA enriched by oligo(dT) beads

SE50/PE100/PE150

mRNA quantification and transcriptome analysis
Eukaryotic total RNA with high integrity
rRNA depleted with rRNA depletion kit
PE100/PE150
lncRNA and transcriptome analysis
Prokaryotic total RNA

rRNA depleted with rRNA depletion kit

PE100/PE150
RNA quantification and transcriptome analysis

Degraded RNA from FFPE samples or plasma cell-free RNA etc. 

rRNA depleted with rRNA depletion kit SE50/PE100
RNA quantification and transcriptome analysis


>

Product Specification

Product name


MGIEasy RNA Directional Library Prep Set


Version V2.0
Reaction/kit

16 RXN (1000006385),96 RXN (1000006386)

Storage temperature requirement

-18℃~-25℃

Input Quantity

10 ng-1 ug total RNA

Sample types

Tissues and FFPE sample

Species Compatibility


Human,animals, plants, fungi and bacteria such as, mouse, rice, Arabidopsis, yeast and E.coli.


Assay Time

7 h
Applications


RNA-Seq, Transcriptome Sequencing, total RNA sequencing, lncRNA Sequencing


Platform Compatibility

BGISEQ-500,DNBSEQ-G400, DNBSEQ-G50, MGISEQ-2000, MGISEQ-200 

Recommended Read Length

SE50/PE100/PE150

Recommended sequencing data per sample

25 M raw reads(SE50)/8 Gb raw data(PE100/PE150)

Recommended MGI Process

Applications
Library preparation 
Sequencing 
Bioinformation analysis
  • Total RNA Sequencing

  • MGISP-100
  • MGISP-960
  • MGIEasy RNA Directional Library Prep Set + MGIEasy rRNA Depletion Kit

  • DNBSEQ-G400/MGISEQ-2000(PE100/ PE150)

  • DNBSEQ-G50/MGISEQ-200(PE100/ PE150)


  • lncRNA Sequencing

  • DNBSEQ-G400/MGISEQ-2000(PE100/ PE150)

  •  DNBSEQ-G50/MGISEQ-200(PE100/ PE150)

>

Related Documents