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Thermo Labile Uracil-DNA Glycosylase

Thermo Labile Uracil-DNA Glycosylase is expressed by a recombinant E. coli strain cloned with UDG gene from Atlantic cod and purified through multiple steps.

UDG (Uracil-DNA Glycosylase) can catalyze the hydrolysis of dU-containing DNA single-stranded or double-stranded uracil base and sugar phosphate backbone N-glycosidic bond, release free uracil, thereby the resulting abasis sites are easily broken by hydrolysis. This product is sensitive to high temperature, and the enzyme can be irreversibly inactivated above 50°C.



Thermo Labile Uracil-DNA Glycosylase

Item No.:1000025346

Thermo Labile Uracil-DNA Glycosylase(1 U/μL)

1 mL/Each

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Thermo Labile Uracil-DNA Glycosylase

Item No.:1000025012

Thermo Labile Uracil-DNA Glycosylase(1 U/μL)

50 μL/Each

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Features

Effectively eliminate uracil
Effectively eliminate uracil
Specifically degrade the dsDNA containing uracil and no affect on dsDNA without uracil
Rapidly inactivate at high temperature
Rapidly inactivate at high temperature
No need to remove , irreversibly inactivated above 50°C
Effectively eliminate uracil
Effectively eliminate uracil
Specifically degrade the dsDNA containing uracil and no affect on dsDNA without uracil
Rapidly inactivate at high temperature
Rapidly inactivate at high temperature
No need to remove , irreversibly inactivated above 50°C

Product Specification

Product Name

Thermo Labile Uracil-DNA Glycosylase

Purity by SDS-PAGE ≥90% pure as determined by SDS-PAGE analysis through Coomassie Blue detection
Optimum Temperature

37℃

Inactivation Temperature&Time

≥50℃, 10 min

Quality UDG (Uracil-DNA Glycosylase) can catalyze the hydrolysis of dU-containing DNA single-stranded or double-stranded uracil base and sugar phosphate backbone N-glycosidic bond, release free uracil, thereby the resulting abasis sites are easily broken by hydrolysis.
Stability

–stored at -20 °C 

Avoid repeated freezing and thawing

Application Eliminate PCR contamination of DNA synthesis reactions.