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RNase Inhibitor

RNase Inhibitor (ribonuclease inhibitor) is a 50 kDa recombinant enzyme used to inhibit RNase activity, protect RNA samples and prevent the degradation of RNA template.The inhibitor specifically inhibits RNases A, B and C. It is not effective against RNase 1, RNase T1, S1 Nuclease,  RNase H or RNase from Aspergillus. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. 

RNase inhibitor is active at pH 5.0-9.0 and 25℃ - 55℃, which is suitable for thermostable reverse transcriptase. It does not contain DNase or endonuclease activity. No inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).



RNase Inhibitor

Item No.:1000014382

RNase Inhibitor (40 U/uL)

2000 U

>

RNase Inhibitor

Item No.:1000015146

RNase Inhibitor (40 U/uL)

40000 U

>

Features

Effectively inhibit RNase A
Effectively inhibit RNase A
Specifically inhibit RNase activity and effectively prevent RNA degradation
Free of DNase or endonuclease activity
Free of DNase or endonuclease activity
Helpful to improve the yield of product
Effectively inhibit RNase A
Effectively inhibit RNase A
Specifically inhibit RNase activity and effectively prevent RNA degradation
Free of DNase or endonuclease activity
Free of DNase or endonuclease activity
Helpful to improve the yield of product

Product Specification

Product Name

RNase Inhibitor

Molecular Weight 50kDa
Purity by SDS-PAGE

≥90% pure as determined by SDS-PAGE analysis through Coomassie Blue detection

Reaction Temperature

25-55℃

Reaction PH 5.0-9.0
Quality

RNAse Inhibitor can efficiently inhibit the activity of RNases A, B and C. 

It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus.

Stability

–stored at -20 °C 

Avoid repeated freezing and thawing

Application

Protect RNA samples 

First strand cDNA synthesis 

In vitro transcription or translation 

RNA library construction 

Single cell RNA library construction