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Alpha Reverse Transcriptase

MGI Alpha Reverse Transcriptase (MGI RT) is an improved enzyme that was developed through in vitro molecular evolution of M-MuLV RT. MGI RT is designed with significantly improved thermostability, enhanced processivity, increased affinity and diminished RNase H activity to enhance consistency and efficiency in cDNA synthesis for RT-PCR and RT-qPCR. 

These features support higher overall cDNA yields with a variety of templates and improved synthesis from templates with complex secondary structures. In RT-qPCR applications, MGI RT also enables high efficiency synthesis over a wide range of input template amounts providing sensitive and accurate quantification of cDNA.



Alpha Reverse Transcriptase

Item No.:1000014383

Alpha Reverse Transcriptase (200U/uL)

10000 U

5 × First Strand Buffer

250 µL

0.1 M DTT

120 µL

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Alpha Reverse Transcriptase

Item No.:1000019050

Alpha Reverse Transcriptase (200U/uL)

200000 U

5 × First Strand Buffer

5 mL

0.1 M DTT

1mL*3

>

Features

Great thermostability
Great thermostability
Up to 60℃
Excellent sensitivity
Excellent sensitivity
cDNA synthesis from total RNA as low as 10 pg; support a wide range of input template (10 pg-1 ug)
High cDNA yield
High cDNA yield
The enzyme ensures high yields of full-length cDNA products up to 11 kb
Low RNase H activity
Low RNase H activity
Decreased by 80.8%, which helps to prevent premature degradation of RNA
Great thermostability
Great thermostability
Up to 60℃
Excellent sensitivity
Excellent sensitivity
cDNA synthesis from total RNA as low as 10 pg; support a wide range of input template (10 pg-1 ug)
High cDNA yield
High cDNA yield
The enzyme ensures high yields of full-length cDNA products up to 11 kb
Low RNase H activity
Low RNase H activity
Decreased by 80.8%, which helps to prevent premature degradation of RNA

Product Specification

Product Name

Alpha Reverse Transcriptase

Purity by SDS-PAGE ≥90% pure as determined by SDS-PAGE analysis through Coomassie Blue detection
Reaction Temperature

42-50℃

Optimum Temperature

42℃

High Temperature Resistance 60℃
Input Quantity 10 pg-1 ug total RNA
Quality

Alpha Reverse Transcriptase is a RT enzyme that was developed through in vitro evolution of M-MuLV RT. 

The engineered enzyme features dramatically improved sensitivity, processivity, and increased affinity compared to wild type M-MuLV RT. 

The enzyme ensures high yields of full-length cDNA products up to 11 kb.

Stability

–stored at -20 °C Avoid repeated freezing and thawing

Application

First strand cDNA synthesis for RT-PCR and RT-qPCR. 

RNA library construction 

Single cell RNA library construction 

Synthesis of full length cDNA for cloning and expression. 

Generation of labeled cDNA probes for microarrays. 

Analysis of RNA by primer extension.