Alpha Reverse Transcriptase
MGI Alpha Reverse Transcriptase (MGI RT) is an improved enzyme that was developed through in vitro molecular evolution of M-MuLV RT. MGI RT is designed with significantly improved thermostability, enhanced processivity, increased affinity and diminished RNase H activity to enhance consistency and efficiency in cDNA synthesis for RT-PCR and RT-qPCR.
These features support higher overall cDNA yields with a variety of templates and improved synthesis from templates with complex secondary structures. In RT-qPCR applications, MGI RT also enables high efficiency synthesis over a wide range of input template amounts providing sensitive and accurate quantification of cDNA.