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MGIEasy Duplex UMI Universal Library Prep Set

High-throughput sequencing plays more important role in cancer screening and drug selection, which is higher depends on low-frequency-variant-detection. Duplex-UMI (unique-molecule-identifier) gets advantage in sensitive and precise sequencing becomes an indispensable tool.

MGIEasy Exome Universal Library Prep Set can be used to prepare library with 10-1000ng segmented input. Duplex barcode is used for calibration, which can effectively reduce the label hopping and contamination between samples. The UMI was used to mark the original molecules, and its correction effect was used to reduce PCR and sequencing errors. This kit adopts high quality enzymatic composition, improved adaptor ligation and high-fidelity enzyme significantly improves the library conversion rate and amplification efficiency. Both FFPE and low input cfDNA are compatiable.



MGIEasy Duplex UMI Universal Library Prep Set (16 rxn)

Item No.:1000018643
Library

MGIEasy Universal DNA Library Prep Module

1000019376

Adapters

MGIEasy Duplex UMI Adapters Kit

1000018645

Clean Beads

MGIEasy DNA Clean Beads

1000005278

Circularization

MGIEasy Dual Barcode Circularization Module

1000018649
>

MGIEasy Duplex UMI Universal Library Prep Set (96 rxn)

Item No.:1000018644
Library

MGIEasy Universal DNA Library Prep Module

1000019377

Adapters

MGIEasy Duplex UMI Adapters Kit

1000018646

Clean Beads

MGIEasy DNA Clean Beads

1000005279

Circularization

MGIEasy Dual Barcode Circularization Module

1000018649
>

Features

 Minimize the contaminate between samples
Minimize the contaminate between samples
Duplex barcode mitigates barcode swapping, minimize the contaminate between samples.
 High conversion rate.
High conversion rate.
10 ng DNA input is allowed for library construction because of high conversion rate.
Ultra-low frequency variant detection
Ultra-low frequency variant detection
Over 200+UMI supports ultra-low frequency variant detection.
 Minimize the contaminate between samples
Minimize the contaminate between samples
Duplex barcode mitigates barcode swapping, minimize the contaminate between samples.
 High conversion rate.
High conversion rate.
10 ng DNA input is allowed for library construction because of high conversion rate.
Ultra-low frequency variant detection
Ultra-low frequency variant detection
Over 200+UMI supports ultra-low frequency variant detection.

Product Specification

Product

MGIEasy Duplex UMI Universal Library Prep Set

Lot.
V1.0
Version

16RXN (1000008643) & 96RXN (1000018644)

Frag. method   

Ultrasound except cfDNA

Library size
200 -600 bp
Assay time ~7 hours
Hands-on time
~30 minutes
Sample requirements

Human genome DNA, including Blood, tissue, FFPE, cfDNA

Suitable Hybridization Probes

MGI, Agilent, Nimblegen, IDT or seminar Probes from other vendors

Sequencing Platform
DNBSEQ-G400, DNBSEQ-G50, MGISEQ-200、MGISEQ-2000、DNBSEQ-T7
Read length
PE100, PE150

Recommended MGI Process

Applications
Library preparation
Sequencing
Analysis

Low frequency variant detection

  • MGISP-100
  • MGISP-960
  • MGIEasy Exome Universal Library Prep Set
  • MGI, Agilent, Nimblegen, IDT or seminar Probes from other vendors
  • DNBSEQ-G50/MGISEQ-200(PE100、PE150)
  • DNBSEQ-G400/MGISEQ-2000(PE100、PE150)
  • DNBSEQ-T7 (PE100、PE150)
  • /
>

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