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CoolMPS High-throughput Sequencing Set

CoolMPS High-throughput Sequencing Set is a novel antibody-based sequencing product. The dNTPs of CoolMPS are without fluorescent labeled (called cold dNTPs) which incorporated into the sequencing strand by DNA polymerase, and base calling is achieved by specific binding of fluorescently labeled antibodies. During this process, the incorporated bases are unmodified, ultimately resulting in clearer base calling.


CoolMPS High-throughput Sequencing Set

CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL SE50)

Item No.:1000017992
1000016985

DNBSEQ-G400RS Sequencing Flow Cell

1000017991

CoolMPS High-throughput Sequencing Kit (DNBSEQ-G400RS FCL SE50)

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CoolMPS High-throughput Sequencing (DNBSEQ-G400RS FCL SE100)

Item No.:1000016933
1000016985

DNBSEQ-G400RS Sequencing Flow Cell


1000016934



CoolMPS High-throughput Sequencing Kit (DNBSEQ-G400RS FCL SE100)


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CoolMPS High-throughput Sequencing (DNBSEQ-G400RS FCL PE100)

Item No.:1000016935
1000016985

DNBSEQ-G400RS Sequencing Flow Cell

1000016936

CoolMPS High-throughput Sequencing Kit (DNBSEQ-G400RS FCL PE100)

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CoolMPS High-throughput Sequencing (DNBSEQ-G400RS Small RNA FCL SE50)

Item No.:1000019478
1000016985

DNBSEQ-G400RS Sequencing Flow Cell

1000017991

CoolMPS High-throughput Sequencing Kit (DNBSEQ-G400RS FCL SE50)

1000006387

MGIEasy Wash Buffer For Small RNA Sequencing

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Features

Labeled antibodies lead to higher signal
Labeled antibodies lead to higher signal
Multiple labels per antibody, stronger signals
No quenching in CoolMPS
No quenching in CoolMPS
Natural base, no “scar” induced quenching
Polymerase provided natural base environment for binding and incorporation, reaction closer to completeness
Polymerase provided natural base environment for binding and incorporation, reaction closer to completeness
Closer to 100% reaction>>Less signal decay>>Great potential for longer reads
Labeled antibodies lead to higher signal
Labeled antibodies lead to higher signal
Multiple labels per antibody, stronger signals
No quenching in CoolMPS
No quenching in CoolMPS
Natural base, no “scar” induced quenching
Polymerase provided natural base environment for binding and incorporation, reaction closer to completeness
Polymerase provided natural base environment for binding and incorporation, reaction closer to completeness
Closer to 100% reaction>>Less signal decay>>Great potential for longer reads

Recommended Applications

Type Part number
Kit name

Pair end sequencing (PE100) for MGI dual index library

1000011532
CPAS Barcode Primer 3
1000016935

CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL PE100)

Single end sequencing (SE50) for App-A single index library

1000017992

CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL SE50)

1000012291

High-Throughput Single-End Sequencing Primer

 Kit (App-A)

Single end sequencing (SE100) for App-A single index library


1000016933

CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL SE100)

1000012291

High-Throughput Single-End Sequencing Primer Kit (App-A)

Pair end sequencing (PE100) for App-A single index library


1000016935

CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL PE100)

1000004156

High-Throughput Pair-End Sequencing Primer Kit (App-A)

Pair end sequencing (PE100) for App-A dual index library


1000016935

CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL PE100)

1000004156

High-Throughput Pair-End Sequencing Primer Kit (App-A)

1000014047

High-Throughput Barcode Primer 3 Reagent Kit(App-A)

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Product Specification

Flow cell
FCL

Read length 

SE50

SE100 PE100
Maximum cycles

70

120 220
Effective reads/flow cell

1500~2000M

1500~2000M 1500~2000M
Data output/flow cell
75~100G 150~200G 300~400G
Q30* >90% >90% >90%
Run time**
~17H ~30H ~58H


*    Effective Reads andQ30 are based on the standard library. These values may vary depending on

various applications.


**   Run time includes DNB loading, sequencing, and data process (Fastq writing). Run time may vary for

various instruments.



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