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MGIEasy Exome FS Library Prep Set

MGIEasy Exome FS Library Prep Set V2.0 aims to prepare libraries with human gDNA. DNA is fragmented by enzyme digestion, followed by the steps of adaptor ligation at both ends and PCR amplification. The amplified PCR product can be used for exome capture with MGIEasy Exome Capture V4/V5 Probe Set or other commercialized probes.



MGIEasy Exome FS Library Prep Set V2.0(16 RXN)

Item No.:1000009658

Core library preparation kit

MGIEasy Exome FS Library Prep Kit(16 RXN)

1000005254

Adaptors

MGIEasy DNA Adapters-16 (tube) Kit V1.0(16 RXN,16 barcodes)

1000005284
Clean beads
MGIEasy DNA Clean Beads
1000005278
Circularization
MGIEasy Circularization Kit V2.0
1000005260
Other
MGIEasy Exome Capture Accessory Kit
1000007743
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Features

Compatible with exome probes from various companies
Compatible with exome probes from various companies
The constructed libraries are compatible with probes from Agilent, Nimblegen, IDT, and etc.
Low DNA input
Low DNA input
The DNA input can be as low as 200ng, with human gDNA input 50-400ng.
Compatible with the automation systems
Compatible with the automation systems
Multiple steps can be finished with the automation systems MGISP-100, such as steps from fragmented DNA to pre-PCR, as well as post-capture elution to post-PCR.
Quick to prepare libraries and easy to operate
Quick to prepare libraries and easy to operate
Library preparation total time,hands-on time ~30min.
Compatible with exome probes from various companies
Compatible with exome probes from various companies
The constructed libraries are compatible with probes from Agilent, Nimblegen, IDT, and etc.
Low DNA input
Low DNA input
The DNA input can be as low as 200ng, with human gDNA input 50-400ng.
Compatible with the automation systems
Compatible with the automation systems
Multiple steps can be finished with the automation systems MGISP-100, such as steps from fragmented DNA to pre-PCR, as well as post-capture elution to post-PCR.
Quick to prepare libraries and easy to operate
Quick to prepare libraries and easy to operate
Library preparation total time,hands-on time ~30min.

Recommended Applications

Library preparation set Probes for capture Compatible sequencing Read length Applications
MGIEasy Exome Universal Library Prep Set MGIEasy Exome Capture V4 Probe Set

BGISEQ-500

DNBSEQ-G400

DNBSEQ-G50

MGISEQ-2000

MGISEQ-200

PE100/PE150 Whole exome sequencing
MGIEasy Exome Capture V5 Probe Set PE100/PE150 Whole exome sequencing
Other commercialized probes PE100/PE150 Whole exome sequencing/ target sequencing
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Product Specification

Product name

MGIEasy Exome FS Library Prep Set

Version V2.0

Packaging (Part number)

16 RXN (1000009658)

Storage

-25℃~-18℃,2℃~8℃(Refer to kit label)

Required sample input

50-400ng gDNA;DNA concentration:≥15 ng/μL;intact or mildly degraded DNA;OD260/280=1.8~2.0,OD260/230 >2.0.

Sample types

Tissues, plasma, FFPE samples

Compatible species

Human

Library preparation time

~7 h

Hands-on tim

~30 min

Compatible probes

Probes MGI, Agilent, Nimblegen, IDT

Fragmentation methods

Enzyme digestion (no fragmentation for cfDNA)

Library fragment size

200 -600 bp

Compatible sequencing platforms

BGISEQ-500,MGISEQ-2000, DNBSEQ-G400

Read length

PE100/PE150

Recommended MGI Process

Applications
Library preparation
Sequencing
Bioinformation analysis
  • Whole exome sequencing

  • MGISP-100
  • MGIEasy Exome FS

    Library Prep Set V2.0

  • MGIEasy Exome Capture V4/V5 Probe Set, other commercialized probes

  • BGISEQ500, DNBSEQ-G400, MGISEQ-2000(PE100、PE150)

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