Beyond basic scientific research, MGI is continuously facilitating breakthroughs to better serve agrigenomics researchers and breeders and expand the applications of our sequencing platforms* in molecular breeding. Based on our self-developed multiplex PCR technology, MGI's ATOPlex platform can design specific multiplex PCR primers for targeted genomes, which applies to agriculture and husbandry. It is able to detect multiple targets simultaneously to obtain the sequence with multiple target ge...

The gRNA within CRISPR/Cas has been found to play a key role in reducing off-target effects. Therefore, the redesign and remodeling of gRNAs could help increase the capabilities of CRISPR tools in terms of simplicity, multiplexing, and specificity. To optimize the Cas12f1 gRNA, Do Yon Kim and colleagues identified five potential modification sites (MSs) in the tracrRNA and crRNA sequences for remodeling to produce a powerful and compact CRISPR-Cas12f1 system. Based on data powered by MGI’s ult...

In 2015, a research team of the Korean Institute of Life Sciences and Technology published an article in Nature Methods "Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells," which emphasized the key role of whole-genome sequencing in identifying the off-target effects of CRISPR-Cas technology and proposed the concept of Digenome-seq for the first time. The researchers developed an effective and specific genome-editing tool, CRISPR-Cas12f1, by remodeling t...

The genotype imputation is a basic approach to impute the unobserved genotypes in the data from the genotyping arrays or genome sequencing, which can critically improve the fine mapping of variants in genome-wide association studies. Based on large-scale and specific populations, the haplotype reference panel constructed by whole-genome sequencing data is a basic tool for genotype imputation. However, the main reference panels include databases such as 1000 Genomes Project Phase 3 (1KGP3), Hapl...

In addition, the team has built an open database of single cells from domestic pig brain tissue (http://120.79.46.200:82) to facilitate researchers' access and browsing of the data, which provides extensive resources for future research regarding neural science, evolutionary developmental biology, and regenerative medicine. Cellular compositions of brain tissue in domestic pigs and its heterogeneity: (a) Subject experiments and data generation process. (b) Clustering result of brain tissue cell...

In November 2019, the latest research result "Dissecting primate early post-implantation development using long-term in vitro embryo culture" was completed by the Institute of Translational Medicine of Kunming University of Science and Technology and the Shenzhen BGI Research Published online in the internationally renowned academic journal Science. The article uses single-cell sequencing technology to analyze the important molecular and cellular biological events of primate embryos after ...