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MGIEasy Fast RNA Library Prep Set

The MGIEasy Fast RNA Library Prep Set is a specifically designed RNA rapid library prep product for DNBSEQ^TMsequencers. The library prep set is optimized to convert 10 ng~1 μg of total RNA into a DNA library for gene expression profiling, transcriptome analysis, or detection of pathogenic microorganisms in high-throughput RNA sequencing on DNBSEQ^TMsequencers.

The library prep set is optimized with high quality enzyme and buffer system to combine cDNA second strand synthesis and end repair steps, which greatly shortens the time of library construction. The second strand synthesis is equipped with two kinds of buffers that can be used to prepare a common or directional library according to your needs. dTTP is replaced with dUTP in the strand-specific second strand synthesis buffer so that dUTP is incorporated into the second strand of cDNA. The high-fidelity DNA polymerase used in this kit cannot amplify the DNA template containing uracil, which achieves strand specificity.

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MGIEasy Fast RNA Library Prep Set (16 RXN)

Item No.：940-000890-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast RNA Library Prep Kit (16 RXN) Item No.: 940-000887-00	1
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit (16 barcode,16 RXN) Item No.: 1000022800	1
Clean Beads	MGIEasy DNA Clean Beads (3.2 mL beads) Item No.: 940-001176-00	1

MGIEasy Fast RNA Library Prep Set (96 RXN)

Item No.：940-000889-00

Component Kits	Product Name & Item No.	Quantity
Core library	MGIEasy Fast RNA Library Prep Kit (96 RXN) Item No.：940-000888-00	1
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit B (96 barcode,96 RXN) Item No.：1000022802	1
Clean Beads	MGIEasy DNA Clean Beads (15 mL beads) Item No.：940-001174-00	1

MGIEasy Fast RNA Library Prep Set C (96 RXN)

Item No.：940-001835-00

Component Kits	Product Name & Item No.	Quantity
Core library	MGIEasy Fast RNA Library Prep Kit (96 RXN) Item No.：940-000888-00	1
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit C (96 barcode,96 RXN) Item No.：940-001748-00	1
Clean Beads	MGIEasy DNA Clean Beads (15 mL beads) Item No.：940-001174-00	1

MGIEasy Fast RNA Library Prep Set (192 RXN)

Item No.：940-000961-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast RNA Library Prep Kit (96 RXN) Item No.: 940-000888-00	2
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit A (96 barcode,96 RXN) Item No.: 1000022801	1
PCR primers & Adapter	MGIEasy UDB Primers Adapter Kit B (96 barcode,96 RXN) Item No.: 1000022802	1
Clean Beads	MGIEasy DNA Clean Beads (15 mL beads) Item No.: 940-001174-00	2

Features

Fast library prep workflow Combine the cDNA second strand synthesis, end repair and dA-tailing into one step, greatly shorten the time of library prep time and minimize hands-on time.	Flexible library perp with two library types Provide two kinds of cDNA second strand synthesis buffer, support to prepare a common or directional library.
Excellent performance High uniformity coverage across transcripts, accurate detection of strand orientation, high concordance of gene expression.	Wide range of total RNA input and multiples sample types Compatible with a range of total RNA input (10 ng~1 ug) and multiples sample types, including low-quality RNA, FFPE RNA
Flexible Solutions Adapt to various RNA enrichment methods, sequencing read lengths，two DNB method (universal circularization or OneStep DNB) to meet the needs of different sample types.

Fast library prep workflow

Combine the cDNA second strand synthesis, end repair and dA-tailing into one step, greatly shorten the time of library prep time and minimize hands-on time.

Flexible library perp with two library types

Provide two kinds of cDNA second strand synthesis buffer, support to prepare a common or directional library.

Excellent performance

High uniformity coverage across transcripts, accurate detection of strand orientation, high concordance of gene expression.

Wide range of total RNA input and multiples sample types

Compatible with a range of total RNA input (10 ng~1 ug) and multiples sample types, including low-quality RNA, FFPE RNA

Flexible Solutions

Adapt to various RNA enrichment methods, sequencing read lengths，two DNB method (universal circularization or OneStep DNB) to meet

the needs of different sample types.

Product Specification

Assay time	~ 4 hrs (from gDNA to PCR library)
Hands-On Time	~ 1 hrs
Input quantity	10 ng~1 μg total RNA
Barcode	~288 Unique Dual Barcodes
Sample types	Total RNA from blood, saliva, fresh tissue, FFPE RNA, Plant total RNA, Animal total RNA, Microbial total RNA, Meta RNA
Species compatibility	Human, Animal, Plant, Microbe
Applications	Whole-Transcriptome Sequencing, mRNA Sequencing, Long non-coding RNA Sequencing, RNA-Seq, Metatranscriptome Sequencing
Strand specificity	Support strand-specificity library prep or regular library prep. The second strand synthesis module is equipped with two buffers (dNTP buffer、dUTP buffer) to meet the need for a conventional library or strand-specific library.
Platform compatibility	DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-G99, DNBSEQ-T7, DNBSEQ-T10×4
Recommended read length	SE50/PE100/PE150
Adaptation automation platform	MGISP-100(16RXN), MGISP-960(96RXN, 192RXN), MGISP-Smart8

Recommended MGI Process

Applications	Library preparation	Sequencing	Data analysis
Whole-Transcriptome Sequencing（Human）	MGISP-100/MGISP-960 RNA enrichment: MGIEasy rRNA Depletion Kit library: MGIEasy Fast RNA Library Prep Set MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-G99 MGISEQ-200 DNBSEQ-T7 DNBSEQ-T10×4
mRNA Sequencing	MGISP-100/MGISP-960 RNA enrichment: other commercial oligo dT beads reagent products library: MGIEasy Fast RNA Library Prep Set MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-G99 MGISEQ-200 DNBSEQ-T7 DNBSEQ-T10×4
Metatranscriptome Sequencing	MGISP-100/MGISP-960 RNA enrichment: other commercial rRNA depletion reagent products for microorganisms library: MGIEasy Fast RNA Library Prep Set MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-G99 MGISEQ-200 DNBSEQ-T7 DNBSEQ-T10×4	Platform of microorganisms Fast Identification (PFI)

Applications

Library preparation

Sequencing

Data analysis

Whole-Transcriptome

Sequencing（Human）

MGISP-100/MGISP-960

RNA enrichment: MGIEasy rRNA Depletion Kit
library: MGIEasy Fast RNA Library Prep Set
MGIEasy Dual Barcode Circularization Kit

DNBSEQ-G400
DNBSEQ-G99
MGISEQ-200
DNBSEQ-T7
DNBSEQ-T10×4

mRNA Sequencing

MGISP-100/MGISP-960
RNA enrichment: other commercial oligo dT beads reagent products

library: MGIEasy Fast RNA Library Prep Set
MGIEasy Dual Barcode Circularization Kit

DNBSEQ-G400
DNBSEQ-G99
MGISEQ-200
DNBSEQ-T7
DNBSEQ-T10×4

Metatranscriptome Sequencing

MGISP-100/MGISP-960
RNA enrichment: other commercial rRNA depletion reagent products for microorganisms
library: MGIEasy Fast RNA Library Prep Set
MGIEasy Dual Barcode Circularization Kit

DNBSEQ-G400
DNBSEQ-G99
MGISEQ-200
DNBSEQ-T7
DNBSEQ-T10×4

Platform of microorganisms Fast Identification (PFI)

Related Products

MGIEasy Dual Barcode Circularization Kit

Application: Prepare the double-stranded DNA library with MGl dual barcode adapter into a single-stranded circular DNA library for DNBSEQ seouencers. Applicable to the double-stranded DNA ibraries with MG1 dual barcode adapter from all MGl dual barcode librarv prep kits.

Platform Compatibility: All DNBSEQ

Reaction/kit: 16 RXN

MGIEasy rRNA & Globin Depletion Kit V1.3

Application: Efficiently deplete rRNA and Globin mRNA from human blood total RNA and keep other mRNA and non-coding RNA.

Reaction/kit: 16 RXN, 96 RXN

MGIEasy rRNA Depletion V1.3

Application: Efficiently deplete rRNA from human, mouse and rat total RNA and keep mRNA and non-coding RNA.