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MGIEasy PCR-Free DNA Library Prep Set

MGIEasy PCR-Free Library Prep Set is specifically designed for making WGS libraries for MGI High-throughput Sequencing Platforms. This library prep set is optimized to convert 120-200 ng size selected fragmented DNA into a customized library. MGI WGS PCR-free library preparation, combined with MGI DNBSEQTM sequencer, is the true PCR-free NGS workflow without PCR amplification, resulting in no error accumulation and higher data accuracy.

Highlight

Compatible with Multiple Species Types:  Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva, fresh tissue), mice, rice, E.coli and metagenomics.
Fast, Simple and Automated Workflow:   Library preparation can be completed in 3.5 hours with size-selected DNA as input; the workflow is simple and suitable for automated library preparation without the risk of amplicon contamination.
No Amplification Error Accumulation:  By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity.
Higher Coverage Uniformity:   Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region, promoter and repetitive region.
Higher Performance of Variant Detection:  Compared with traditional WGS (PCR amplification), PCR-free WGS shows higher sensitivity and accuracy of variant detection, especially indels.

Specification

Product Name
MGIEasy PCR-Free DNA Library Prep Set
Application
Whole genome sequencing
The version of Set V1.0
PN.

1000013452,1000013453

Configuration
16 RXN, 96 RXN
Validity period
9 months
Assay Time
~3.5 hours
Input Quantity
120-200 ng fragment DNA
Sample types
gDNA
Species Compatibility

Human, animals,plants, fungi, bacteria, metagenomics, etc.

Fragmentation Method
Sonication
Recommended Insert Size
350-400 bp
Platform Compatibility
BGISEQ-500,MGISEQ-2000
Recommended Read Length
PE100,PE150
Instruction
MGIEasy PCR-Free DNA Library Prep Set User Manual

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