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MGIEasy RNA Directional Library Prep Set
MGIEasy RNA Directional Library Prep Set provides an efficient solution for generating libraries suitable for MGI high-throughput sequencing platforms from total RNA. It can be used to identify RNA stranded information that from which of the two DNA strands a given RNA transcript originates. Identifying strand origin increases the percentage of reads that align to the transcriptome and provides more accurate information for studies in regulation of gene expression and gene functional analysis. Maintaining strand orientation also allows identification of antisense expression and novel genes.
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Features

Wide range of total RNA input
Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.
Compatible with various sample types
Including low-quality RNA, FFPE RNA and plasma samples
Excellent coverage quality
Accurate and comprehensive mapping of transcripts
High uniformity
Enables high uniform from transcripts 5 'to 3' ends and superior 3’end coverage
Accurate detection of strand orientation
Identify transcript origin, detect antisense transcripts, enhances transcript annotation
Easy to Use
Simple protocol and short operation time, suitable for automated library preparation instrument
Flexible Solutions
Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types

Applications

Sample type RNA enrichment method Read length Applications
Eukaryotic total RNA with high integrity Poly(A)+ mRNA enriched by oligo(dT) beads SE50/ PE100/ PE150 mRNA quantification and transcriptome analysis
Eukaryotic total RNA with high integrity rRNA depleted with rRNA depletion kit PE100/ PE150 lncRNA and transcriptome analysis
Prokaryotic total RNA rRNA depleted with rRNA depletion kit PE100/ PE150 RNA quantification and transcriptome analysis
Degraded RNA from FFPE samples or plasma cell-free RNA etc. rRNA depleted with rRNA depletion kit SE50/ PE100 RNA quantification and transcriptome analysis

Specification

Product name MGIEasy RNA Directional Library Prep Set
Version V2.0
Reaction/Set 16 RXN (1000006385)\ 96 RXN (1000006386)
Storage temperature requirement -18℃~-25℃
Input Quantity 10 ng-1 ug of total RNA
Sample types tissues, plasma and FFPE sample
Species Compatibility Human, animals, plants, fungi and bacteria, such as mouse, rice, Arabidopsis, yeast and E.coli.
Assay Time 7 h
Applications RNA-Seq, Transcriptome Sequencing, total RNA sequencing, lncRNA Sequencing
Platform Compatibility BGISEQ-500, MGISEQ-2000 x
Recommended Read Length SE50/PE100/PE150
Recommended sequencing data per sample 25 M raw reads(SE50)/8 Gb raw data(PE100/PE150)

Files

Data Sheet MGIEasy RNA directional library prep set Download
User Manual 1000006385, 1000006386-MGIEasy RNA Directional Library Prep Set User Manual Download

This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used.
Demo Data Read Length: PE150      Sample: UHRR      Platform: MGISEQ-2000

I    Sample Information         Fastq Download
II   Sample Information         Fastq Download
III  Sample Information          Fastq Download
Ⅳ  Sample Information         Fastq Download
Ⅴ  Sample Information         Fastq Download
Ⅵ  Sample Information         Fastq Download

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