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MGIEasy FS PCR-Free DNA Library Prep Set


MGIEasy FS PCR-Free Library Prep Set is specifically designed for making WGS libraries without PCR for MGI High-throughput Sequencing Platforms. This library prep set could fragment 500 - 1000 ng gDNA into fragment DNA by high-quality fragmentation enzyme and prepare the library for MGI sequencing platforms. MGI WGS PCR-free library preparation, combined with MGI DNBSESQTM sequencer,is the true PCR-free NGS workflow without PCR amplification, resulting in no error accumulation and higher data accuracy.



Highlight

Compatible with Multiple Species Types:Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva,fresh tissue), mice, rice, E.coli and metagenomics.

Fast, Simple and Automated Workflow :Automated library preparation process can be completed in 4.5 hours

No Amplification Error Accumulation: By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQTM platform has no amplification error accumulation, guaranteeing the utmost fidelity of genome sequence.

Higher Coverage Uniformity :Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region,promoter and repetitive region.

Excellent Performance of Variant Detection: Compared with traditional WGS (PCR amplification),WGS PCR-free shows higher sensitivity and precision of variant detection, especially indels.

Specification

Product Name
MGIEasy FS PCR-Free DNA Library Prep Set
Application
Whole genome sequencing
The version of Set V1.0
Cat.

1000013454,

1000013455

Configuration
16 RXN, 96 RXN
Validity period
9 months
Assay Time
~4.5 hours
Input Quantity
500 ng - 1 μg gDNA
Sample types
gDNA
Species Compatibility

Human, animals,plants, fungi, bacteria, metagenomics, etc.

Fragmentation Method
Fragmentation enzyme
Recommended Insert Size
350-475 bp
Platform Compatibility
MGISEQ-2000,BGISEQ-500
Recommended Read Length
PE100, PE150
Instruction

MGIEasy FS PCR-Free DNA Library Prep Set User Manual



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