Reagent Products
ALL library preparation
MGIEasy Exome Universal Library Prep Set

The MGIEasy Exome Universal Library Prep Set operate with gDNA followed by CovarisTM Focused-ultrasonicator fragmentation, MGI adapter ligation and amplification. The PCR product is applied to hybrid capture with MGIEasy Exome Capture Universal Set or other commercial exome capture probe. Captured product is amplified for the second time and denatured for the single stranded circle preparation which is ready for MGI high-throughput sequencing platform.

Features

Comparable to multiple whole exon probe

Automatic operation available for key library construction process

Simple operation, short time for library construction

Specification

Product name

MGIEasy Exome Universal Library Prep Set

PN. 1000009657

Assay Time

~7 hours

Hands-On Time

~30 min

Input Quantity

1 μg gDNA;

OD260/280=1.8~2.0;

DNA concentration≥12.5 ng/μL


Sample types

Tissues, Plasma and FFPE sample

Compatible Probe Brand


MGI, Agilent, NimbleGen, IDT


Recommended Read Length

PE100/PE150

Fragment Process

Ultrasonic

Recommended Insert Size

100 – 500 bp

Platform Compatibility

MGISEQ-2000、MGISEQ-200、BGISEQ-500

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