Preimplantation Genetic Screening (PGS) is a very important assisted reproductive technology today, but it also faces the problems of cumbersome operation and inefficiency. To improve the process, MGI has developed a highly automated PGS solution to help achieve efficient and stable local PGS services. MGI has released its new PGS solution globally at the European Society of Human Reproduction and Embryology (ESHRE) 2019 conference on June 25, advancing the science of genetic screening for human reproduction.
In recent years, as the incidence of infertility has increased, the clinical application of assisted reproductive technology has become more common. Improving the success rate and live birth rate of embryo implantation is a major goal in the treatment cycle of assisted reproductive technology.
Multiple studies have shown that Preimplantation Genetic Screening (PGS) can be used to select embryos without copy number variation to implant into the uterine cavity, which reduces the rate of miscarriage, shortens the conception cycle, and improves the embryo implantation success rate and subsequent live birth. PGS can also increase the proportion of single embryo transfer and reduce the risk of twin or multiple pregnancies. At present, there are a variety of PGS detection technologies and services in the clinic, but there are problems such as cumbersome operation, uneven automation, and instability caused by sample or data transportation.
To this end, MGI has created a one-stop total solution for future PGS applications, covering the entire process required for the entire test, including WGA, library preparation, high-throughput sequencing, data analysis, and embryo copy number variation report. The advent of MGI's PGS overall solution will greatly promote the localization process of efficient PGS detection services.
Introducing PGS overall solution
The program is based on the flexible and efficient small genome sequencing platform MGISEQ-200. It is composed of MGISP-100 automated sample preparation system, Single Cell CNV data analysis and report interpretation system, and ZLIMS information management platform specially designed for the sequencing process. A complete solution from embryonic cell to output copy number variation detection report, with low initial sample amount, no contamination, high library quality, consistent results, space saving design, etc., MGI’s PGS one-stop solution can fully meet the needs for localization of PGS screening service.
The component module
1. MGISEQ-200 sequencer
The MGISEQ -200 is a focused, highly efficient small genome sequencer for multi-applications targeting DNA and RNA sequencing, microbial detection, small genome sequencing, etc. The machine supports multiple read lengths and optimizes different read lengths. The sequencing time can flexibly meet the throughput and time requirements of different application scenarios.
2. MGISP-100 Automated Sample Preparation System
The MGISP-100 is an automated sample preparation system focused on high-throughput sequencing, which is fast, stable and efficient and can replace a series of experimental operations for manual library construction. The integrated design of the MGISP-100 enables the preparation of 16 samples at a time, providing a one-stop solution for complex database construction steps.
3. Single Cell CNV Data Analysis and Report Interpretation System
Single Cell CNV is a single-cell copy number variation analysis software independently developed by MGI. It optimizes the integration process from embryonic cell amplification to generation of detection and identification reports, and it can quickly detect more than 4M deletions in chromosomes, as well as repeats and aneuploidy.
4. ZLIMS System
ZLIMS is an information management platform designed for the sequencing process. It can record experimental process data, monitor the real-time status of instruments and samples, schedule offline data to storage, integrate information analysis platforms, initiate automated analysis, and form a sample entry to final report, for an end-to-end complete solution.
Our unique advantages:
1. Compatible with low DNA input
This total solution overcomes the difficulty of working with low DNA input and is sensitive enough for DNA input as low as 6.6 pg for a single cell.
2. Stable and contamination-free
High-throughput sequencing technology has detailed and complicated operation steps and requires significant manpower. In order to free up staff and save space, the introduction of automated equipment enables complex high-throughput sequencing to be completed at the assisted reproductive center. At the same time, due to the low initial amount of PGS detection, efficient anti-contamination measures are required. The PGS overall solution provides a closed working space. The high-efficiency HEPA filter system provides positive pressure filtration air for the pipetting space in the workstation. UV disinfection before and after the experiment can effectively avoid contamination on the experiment, producing high quality and highly reproducible results.