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ALL library preparation Sequencing Kit
MGIEasy RNA Exome Application
MGIEasy RNA Exome is an efficient solution for targeted quantification of the coding regions of the human transcriptome. By combining directional library prep and exome capture technologies, it involves conversion of total RNA into cDNA while keeping the strand direction information, targeted capture of exon sequences, and generation of libraries for sequencing on MGISEQ platforms. MGIEasy RNA Exome provides an efficient and complete solution for mRNA expression quantification of FFPE and other types of degraded samples.
MGIEasy RNA Exome is designed specifically for degraded RNA samples, by capturing only the coding regions from total RNA, it can help improve the accuracy of mRNA quantification. It requires much less data output to achieve the same required sequencing depth for the target coding regions.
MGIEasy RNA Exome Solution includes MGIEasy RNA Directional Library Prep Set, MGIEasy Exome Capture V5 Probe Set, and MGIEasy Exome Capture Accessory Kit. This guide will introduce the details of MGIEasy RNA Exome library preparation technology.
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Features

Low input amount
As little as 10 ng total input RNA. Libraries show stable performance across various input amounts.
Suitable for degraded samples
High quality data can still be obtained for FFPE and other types of degraded samples.
Focus on the RNA coding region
Targeted capture of human exome regions from total RNA means less data output required to achieve the same sequencing depth at the target regions.
High repeatability
The repeatability for low quality samples is above 0.99.

Specification

Application name MGIEasy RNA Exome
Instruction MGIEasy RNA Exome Instructions
Item information MGIEasy RNA Directional Library Prep Set (1000006385) MGIEasy Exome Capture V5 Probe Set(1000007746) MGIEasy Exome Capture Accessory Kit(1000007743)
Reaction/kit 16 RXN
Input Quantity 10 ng-100 ng total RNA
Sample types Fresh Frozen tissues, FFPE samples
Species Compatibility Human
Assay Time 32 h
Applications RNA-Seq
Platform Compatibility BGISEQ-500,DNBSEQ-G400, MGISEQ-2000
Recommended Read Length PE100
Recommended sequencing data per sample PE100 / 25M reads

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