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MGIEasy FS PCR-Free DNA Library Prep Set


MGIEasy FS PCR-Free Library Prep Set is specifically designed for making WGS libraries without PCR for MGI High-throughput Sequencing Platforms. This library prep set could fragment 200 - 1000 ng gDNA into fragment DNA by high-quality fragmentation enzyme and prepare the library for MGI sequencing platforms. MGI WGS PCR-free library preparation, combined with MGI DNBSESQTM sequencer,is the true PCR-free NGS workflow without PCR amplification, resulting in no error accumulation and higher data accuracy.

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Compatible with Multiple Species Types:Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva,fresh tissue), mice, rice, E.coli and metagenomics.

Fast, Simple and Automated Workflow :Automated library preparation process can be completed in 4.5 hours

No Amplification Error Accumulation: By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQTM platform has no amplification error accumulation, guaranteeing the utmost fidelity of genome sequence.

Higher Coverage Uniformity :Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region,promoter and repetitive region.

Excellent Performance of Variant Detection: Compared with traditional WGS (PCR amplification),WGS PCR-free shows higher sensitivity and precision of variant detection, especially indels.

Specification

Product Name
MGIEasy FS PCR-Free DNA Library Prep Set
Application
Whole genome sequencing
The version of Set V1.0
Cat.

1000013454,

1000013455

Configuration
16 RXN, 96 RXN
Validity period
9 months
Assay Time
~4.5 hours
Input Quantity
200 ng - 1 μg gDNA
Sample types
gDNA
Species Compatibility

Human, animals,plants, fungi, bacteria, metagenomics, etc.

Fragmentation Method
Fragmentation enzyme
Recommended Insert Size
350-475 bp
Platform Compatibility
MGISEQ-2000,BGISEQ-500
Recommended Read Length
PE100, PE150
Instruction

MGIEasy FS PCR-Free DNA Library Prep Set User Manual



Files

User Manual 1000013454,1000013455 MGIEasy FS PCR-Free DNA Library Prep Set User Manual Download

This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used.
Demo Data Read Length:PE150    Sample:NA12878    Platform:MGISEQ-2000

I Sample Information      Fastq Download
II Sample Information     Fastq Download
III Sample Information    Fastq Download
IV Sample Information    Fastq Download

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