|Product name||MGIEasy stLFR Library Prep Kit|
|Current status||Coming soon. Now recruiting beta testing volunteers|
|Shelf life||Six months|
MGIEasy stLFR Library Prep Kit（Box 1）
MGIEasy stLFR Library Prep Kit（Box 2）
|Shipping condition||Dry ice|
|Input material||Genome DNA|
|Species supported||Multiple species|
|Type of samples||High molecular weight DNA|
|Requirements for DNA sample||
The DNA required for stLFR library needs to be as large as it can be. The larger the DNA is the better the performance will be.
Usually the DNA isolated with methods suggested below will be with a main band greater than 100 kb when analyzing with pulse-field electrophoresis.
The suggested DNA isolated kits are Qiagen MagAttract HMW DNA Kit (Cat No./ID: 67563) or Agilent Technologies RECOVEREASE DNA ISOLATION KIT (Catalog No.50-126-011)
The required concentration of DNA should be more than1 ng/ul and the total amount should be more than 500ng.
|Positive control||NA12878 （CORIELL，NA12878）|
|Library process||Shear DNA with enzyme, perform DNA co-barcoding, introduce another adapter, amplify with PCR and make DNB|
|Sequencing read length suggested||PE100+42|
|Sequencing platforms suggested||BGISEQ-500RS、MGISEQ-2000RS|
|Suggested coverage/Raw reads number||For human resequencing purpose: 30X， ~650M raw reads (PE100)|
|User Guidance||MGIEasy stLFR Library Prep kit User Guidance|