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MGIEasy stLFR Library Prep Kit*

MGIEasy stLFR Library Prep Kit* is the world’s first partition-less long fragment DNA co-barcoding library prep kit [1]. Based on the principle of DNA co-barcoding, which adds the same barcode sequence to sub-fragments of the original long DNA molecule, stLFR achieves long range genetic information using high throughput and highly precise short read sequencing. Coupled with the MGI DNBSEQTM sequencing platform, stLFR enables high quality small variants calling, phasing of over 99% of the human genome, better genome coverage, detection of large structural variants, and other long read applications.



[1] - Wang et al, 2019. Genome Res.29: 798-808.


* This Product is Only for Sale in the United States, and for Research Use Only.




MGIEasy stLFR Library Prep Kit*

MGIEasy stLFR Library Prep Kit (16 rxn)

Item No.:940-000193-00
>

MGIEasy stLFR Library Prep Kit (48rxn)

Item No.:940-000194-00
>

Features

√Long range information
√Long range information
Long fragment read lengths of 50-70kb on average, up to 300kb
√Ultra-low DNA input
√Ultra-low DNA input
Only 1.5ng required, 98% less than single molecule sequencing methods
√Haplotype phasing
√Haplotype phasing
Derive heterozygous variants linkage information for genetic disease and reproductive health applications
√Single-tube reaction
√Single-tube reaction
No need for nanoliter liquid handling or additional purchase of microfluidic systems
√Automatable library prep
√Automatable library prep
Automatable library prep on the MGISP-960, minimal hands-on time and effort
√Structural variants detection
√Structural variants detection
Detect copy number variants, deletions, inversions, and balanced translocations.
√Long range information
√Long range information
Long fragment read lengths of 50-70kb on average, up to 300kb
√Ultra-low DNA input
√Ultra-low DNA input
Only 1.5ng required, 98% less than single molecule sequencing methods
√Haplotype phasing
√Haplotype phasing
Derive heterozygous variants linkage information for genetic disease and reproductive health applications
√Single-tube reaction
√Single-tube reaction
No need for nanoliter liquid handling or additional purchase of microfluidic systems
√Automatable library prep
√Automatable library prep
Automatable library prep on the MGISP-960, minimal hands-on time and effort
√Structural variants detection
√Structural variants detection
Detect copy number variants, deletions, inversions, and balanced translocations.

Product Specification

Product Name

MGIEasy stLFR Library Prep Kit*

Specification(Product No.)

16 RXN (940-000193-00), 48 RXN(940-000194-00)

Shelf life
12 months
Input material

Genomic DNA

Species supported
Multiple species
Type of samples
High molecular weight DNA
Requirements for DNA sample

Sample concentration should be more than 1 ng/μL, the A260/A280 value of the DNA sample should be 1.6 to 2.2. The mean length of the initial DNA fragments should be longer than 40 Kb with no degradation below 40 Kb. DNA fragments with longer length will produce better results.

Library prep process

Enzymatic shearing, co-barcoding of long DNA fragments by bead capture and ligation, PCR amplification, circularization, and make DNB.

Compatible sequencing platforms

DNBSEQ-G400, DNBSEQ-T7

Sequencing read length

PE100+42+10

Recommended coverage/ number of raw reads


For human resequencing purpose (30x): 130Gb or 650M PE100 raw reads



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  • *For research use only.

    Not for use in diagnostic procedures (except as specifically noted).
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