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MGIEasy RNA Directional Library Prep Set
MGIEasy RNA Library Prep Set provides an efficient solution for generating libraries from a wide range (10 ng-1 ug) of total RNA that is suitable for MGI high-throughput sequencing platforms. It is easy to use and can be widely applied to human, animal, plant and microbe transcriptome research for comprehensive and rapid analysis of gene expression changes, examining rare and novel transcripts, discovery of alternate splicing events, gene fusions, SNPs and allele-specific expression.
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Features

Wide range of total RNA input
Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.
Compatible with various sample types
Including low-quality RNA, FFPE RNA and plasma samples
Excellent coverage quality
Accurate and comprehensive mapping of transcripts
High uniformity
Enables high uniform from transcripts 5 'to 3' ends and superior 3’end coverage
Accurate detection of strand orientation
Identify transcript origin, detect antisense transcripts, enhances transcript annotation
Easy to Use
Simple protocol and short operation time, suitable for automated library preparation instrument
Flexible Solutions
Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types

Applications

Sample type RNA enrichment method Read length Applications
Eukaryotic total RNA with high integrity Poly(A)+ mRNA enriched by oligo(dT) beads SE50/ PE100/ PE150 mRNA quantification and transcriptome analysis
Eukaryotic total RNA with high integrity rRNA depleted with rRNA depletion kit PE100/ PE150 lncRNA and transcriptome analysis
Prokaryotic total RNA rRNA depleted with rRNA depletion kit PE100/ PE150 RNA quantification and transcriptome analysis
Degraded RNA from FFPE samples or plasma cell-free RNA etc. rRNA depleted with rRNA depletion kit SE50/ PE100 RNA quantification and transcriptome analysis

Specification

Product name MGIEasy RNA Directional Library Prep Set
Version V2.0
Reaction/Set 16 RXN (1000006385)\ 96 RXN (1000006386)
Storage temperature requirement -18℃~-25℃
Input Quantity 10 ng-1 ug of total RNA
Sample types tissues, plasma and FFPE sample
Species Compatibility Human, animals, plants, fungi and bacteria, such as mouse, rice, Arabidopsis, yeast and E.coli.
Assay Time 7 h
Applications RNA-Seq, Transcriptome Sequencing, total RNA sequencing, lncRNA Sequencing
Platform Compatibility BGISEQ-500, MGISEQ-2000 x
Recommended Read Length SE50/PE100/PE150
Recommended sequencing data per sample 25 M raw reads(SE50)/8 Gb raw data(PE100/PE150)

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