Reagent Products
Reagent supplies
ALL library preparation
MGIEasy RNA Directional Library Prep Kit
MGIEasy RNA Directional Library Prep Set provides an efficient solution for generating libraries suitable for MGI high-throughput sequencing platforms from total RNA. It can be used to identify RNA stranded information that from which of the two DNA strands a given RNA transcript originates. Identifying strand origin increases the percentage of reads that align to the transcriptome and provides more accurate information for studies in regulation of gene expression and gene functional analysis. Maintaining strand orientation also allows identification of antisense expression and novel genes.
File Download

Features

Wide range of total RNA input
Compatible with a range of total RNA input (0.2-2.5 ug) and exhibits excellent quality sequencing data and transcript annotation.
Compatible with various sample types
Including low-quality RNA, FFPE RNA and plasma samples
Excellent coverage quality
Accurate and comprehensive mapping of transcripts
High uniformity
Enables high uniform from transcripts 5 'to 3' ends and superior 3’end coverage
Accurate detection of strand orientation
Identify transcript origin, detect antisense transcripts, enhances transcript annotation
Easy to Use
Simple protocol and short operation time, suitable for automated library preparation instrument
Flexible Solutions
Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types

Applications

Sample type RNA enrichment method Read length Applications
Eukaryotic total RNA with high integrity Poly(A)+ mRNA enriched by oligo(dT) beads SE50/ PE100/ PE150 mRNA quantification and transcriptome analysis
Eukaryotic total RNA with high integrity rRNA depleted with rRNA depletion kit PE100/ PE150 lncRNA and transcriptome analysis
Prokaryotic total RNA rRNA depleted with rRNA depletion kit PE100/ PE150 RNA quantification and transcriptome analysis
Degraded RNA from FFPE samples or plasma cell-free RNA etc. rRNA depleted with rRNA depletion kit SE50/ PE100 RNA quantification and transcriptome analysis

Specification

Product name MGIEasy RNA Directional Library Prep Kit
Version V1.0
Reaction/kit 32 RXN/kit
Catalog No. 1000003977 -
Storage temperature requirement 18℃~-25℃
Input Quantity -18℃~-25℃ 0.2-2.5 ug of total RNA
Sample types tissues, plasma and FFPE sample
Species Compatibility Human, animals, plants, fungi and bacteria, such as mouse, rice, Arabidopsis, yeast and E.coli.
Insert size 150 - 300 bp or 200 - 400 bp
Assay Time 10.5 h
Applications RNA-Seq, Transcriptome Sequencing, total RNA sequencing, lncRNA Sequencing
Platform Compatibility BGISEQ-500, MGISEQ-2000 x
Recommended Read Length SE50/PE100/PE150
Recommended sequencing data per sample 25 M raw reads(SE50)/8 Gb raw data(PE100/PE150)

Contact us

Please leave your contact information to download more product information.
4000-966-988Hot Line
Online serviceOnline service

Wechat Wechat