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MGIEasy mRNA Library Prep Kit
MGIEasy RNA Library Prep Set provides an efficient solution for generating libraries from a wide range (0.2-2.5 ug) of total RNA that is suitable for MGI high-throughput sequencing platforms. It is easy to use and can be widely applied to human, animal, plant and microbe transcriptome research for comprehensive and rapid analysis of gene expression changes, examining rare and novel transcripts, discovery of alternate splicing events, gene fusions, SNPs and allele-specific expression.
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Features

Wide range of total RNA input
Compatible with a range of total RNA input (0.2-2.5 ug) and exhibits excellent quality sequencing data and transcript annotation.
Compatible with various sample types
Including low-quality RNA, FFPE RNA and plasma samples
Excellent coverage
Accurate and comprehensive mapping of transcripts
High uniformity Enables detection of complete transcripts without bias at either end
Easy to Use
Simple protocol and short operation time, suitable for automated library preparation instrument
Flexible Solutions
Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types

Applications

Sample type RNA enrichment method Read length Applications
Eukaryotic total RNA with high integrity Poly(A)+ mRNA enriched by oligo(dT) beads SE50/ PE100/ PE150 mRNA quantification and transcriptome analysisMRNA
Degraded RNA from FFPE samples or plasma cell-free RNA etc. rRNA depleted with rRNA depletion kit SE50/ PE100 RNA quantification and transcriptome analysis

Specification

Product name MGIEasy mRNA Library Prep Kit
Version V2.0
Reaction/kit 32 RXN/kit
Catalog No. 1000005273 -
Storage temperature requirement 18℃~-25℃ 0
Input Quantity .2-2.5 ug of total RNA
Sample types tissues, plasma and FFPE sample
Species Compatibility Human, animals, plants and fungi, such as mouse, rice, Arabidopsis and yeast.
Insert size 150 - 300 bp or 200 – 400 bp
Assay Time 10.5 h
Applications RNA-Seq, Transcriptome Sequencing
Platform Compatibility BGISEQ-500, MGISEQ-2000
Recommended Read Length SE50/PE100/PE150
Recommended sequencing data per sample 25 M raw reads(SE50)/8 Gb raw data(PE100/PE150)

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