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MGI has lauched PE150 for MGISEQ-2000

2018-08-23


MGISEQ-2000 Timeline:

● 2017 October 27: ICG-12 Debuts

● 2018 February 14: Successive delivery to BGI Genomics, Gene+, WeGene and Riken Japan, etc al.

● 2018 Summer: There goes PE150 for MGISEQ-2000!


About PE150 sequencing:

> Average running time: 3 days

> Average effective reads per Flow Cell: up to 1500M

> 4 lanes per Flow Cell

> Average effective reads per lane: at least 350M

> Maximal sequencing cycles: 320


Now open for order!


Data speak for themselves:

Stable Performance

Sample source: E.coli

Validated among various repetitive experiments

Total reads staying over 350M/lane

Q30 consistently above 85%

About PE150 sequencing:

> Average running time: 3 days

> Average effective reads per Flow Cell: up to 1500M

> 4 lanes per Flow Cell

> Average effective reads per lane: at least 350M

> Maximal sequencing cycles: 320


Now open for order!


Data speak for themselves:

Stable Performance

Sample source: E.coli

Validated among various repetitive experiments

Total reads staying over 350M/lane

Q30 consistently above 85%


Reproducibility with the same batch of flowcells, same batch of reagents on one machine. (analyzing 6 lanes)

Total reads CV=3.24%, data quality Q30 CV=1.13%

Reproducibility between 3 reagent batches (analyzing 25 lanes)

Total reads CV=3.34%, data quality Q30 CV=0.93%

Reproducibility between 3 instruments (analyzing 20 lanes)

Total reads CV=2.76%, data quality Q30 CV=0.93%

Reproducibility between 3 batch of flowcells (analyzing 6 lanes)

Total reads CV=4.96%, data quality Q30 CV=1.79%

A wide range of applications

Stable and excellent performance with a variety of libraries tested: whole genome (including PCR-free), transcriptome, region capture, amplicon, tumor panel and more


WGS

Sample source: NA12878

Distribution of MGISEQ-2000 reads base and quality

Q20 97.2%  Q30 91.3%

Summary of WGS performancebetween sequencing platforms

Indel and SNP detection between sequencing platforms

·SNP detection sensitivity is up to 99.48%

·Indel detection sensitivity reaches 95.03%


Transcriptome

Sample source: UHRR

The Genome mapping ratio on the MGISEQ-2000 platform is about 2% higher than the N sequencing platform, and the Gene mapping ratio is about 6% higher than the B library prep kiton the N sequencing platform.


The MGISEQ-2000 platform showed similar results as of the N sequencing platform on Gene number and Transcript number.

Random comparison of reads between sequencing platforms




The randomness of the MGISEQ-2000 platform and the N sequencing platform of the B library prep kitis comparable, and the coverage at the 3' end is better than that of the A library prep kiton the N sequencing platform.

●Of course, the advantages of PE150 reagent for MGISEQ-2000RS high-throughput genetic sequencer are far more than the above.

●Now PE150 is here waiting for you

●Get to know more/order now


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